Immunoblotting: use at 1-2 μg/mL. A band of >200 kDa is detected. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes.
限制
仅限研究用
状态
Liquid
溶解方式
Dilute in PBS or medium that is identical to that used in the assay system.
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
抗原
Ankyrin G (ANK3)
(Ankyrin 3, Node of Ranvier (Ankyrin G) (ANK3))
Ankyrin-3,In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368). {PubMed:17974005}., [Isoform 5]: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin. {PubMed:17974005}.,Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and for at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers, and ion transporters in the plasma membrane.,Cytoplasm, cytoskeleton, Cell projection, axon, Cell membrane, sarcolemma, Cell junction, synapse, postsynaptic cell membrane, Lysosome, Cell membrane, sarcolemma, T-tubule,ANK-3, Ankyrin-G