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ACLY 抗体 (AA 306-502)

Name: ACLY 抗体 (AA 306-502)
Brand: antibodies-online
SKU: ABIN1098143
Availability: OutOfStock

ACLY 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5F8D11 unconjugated

Western blot analysis using ACLY mAb against human ACLY recombinant protein. (ACLY 抗体 (AA 306-502))

Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple). (ACLY 抗体 (AA 306-502))

FACS

Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). (ACLY 抗体 (AA 306-502))

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining. (ACLY 抗体 (AA 306-502))

IHC

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ACLY mouse mAb with DAB staining. (ACLY 抗体 (AA 306-502))

IHC

Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate. (ACLY 抗体 (AA 306-502))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (ACLY 抗体 (AA 306-502))

ELISA

7 images

(2 references)

产品编号 ABIN1098143

发货至: 中国

Datasheet as PDF

抗原 See all ACLY 抗体

ACLY (ATP Citrate Lyase (ACLY))
抗原表位
19

15

7

7

5

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 306-502
适用
91

39

38

8

6

6

4

3

3

3

3

2

2

2

1

1
人
宿主
80

13
小鼠
Compatible Secondaries
克隆类型
72

21
单克隆
标记
46

6

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
This ACLY antibody is un-conjugated
应用范围
74

33

27

26

14

13

12

12

7

6

3

3

3

2
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

原理

ACLY Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human ACLY (AA: 306-502 ) expressed in E. Coli.

克隆位点

5F8D11

亚型

IgG1
anti-ATP Citrate Lyase (ACLY) (AA 752-1101) antibody
ACLY 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (pSer455) antibody
ACLY 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (AA 306-502) antibody
ACLY 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (AA 4-265) antibody
ACLY 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) antibody
ACLY 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal 3A5 unconjugated Recombinant Antibody

anti-ATP Citrate Lyase (ACLY) (C-Term) antibody
ACLY 适用: 人, 小鼠 WB, FACS, ICC 宿主: 小鼠 Monoclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (Middle Region) antibody
ACLY 适用: 人, 小鼠, 大鼠, Pig, Cow, 斑马鱼, 兔, 绵羊, 犬, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) antibody
ACLY 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (Ser1227) antibody
ACLY 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-ATP Citrate Lyase (ACLY) (AA 793-1008) antibody
ACLY 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Chu, Lin, Hendel, Kulpa, Brownsey, Johnson: "ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells." in: The Journal of biological chemistry, Vol. 285, Issue 42, pp. 32606-15, (2010) (PubMed).

Beckner, Fellows-Mayle, Zhang, Agostino, Kant, Day, Pollack: "Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas." in: International journal of cancer. Journal international du cancer, Vol. 126, Issue 10, pp. 2282-95, (2010) (PubMed).
抗原

ACLY (ATP Citrate Lyase (ACLY))

别名

ACLY (ACLY 产品)

别名

ACLY antibody, DDBDRAFT_0205389 antibody, DDBDRAFT_0235360 antibody, DDB_0205389 antibody, DDB_0235360 antibody, ACL antibody, BcDNA:LD21334 antibody, CG8322 antibody, DmATPCL antibody, Dmel\\CG8322 antibody, anon-WO0140519.179 antibody, dATPCL antibody, l(2)01466 antibody, l(2)k09217 antibody, n(2)k09217 antibody, ATPCL antibody, CLATP antibody, A730098H14Rik antibody, AW538652 antibody, Clatp antibody, acly antibody, cb722 antibody, zgc:92008 antibody, ATP citrate lyase antibody, ATP citrate lyase S homeolog antibody, ATP-citrate synthase antibody, citrate synthase, mitochondrial antibody, atp-citrate synthase antibody, ATP citrate lyase a antibody, ACLY antibody, acly.S antibody, LOC587157 antibody, acly antibody, ATPCL antibody, LOC5564509 antibody, CpipJ_CPIJ010291 antibody, Bm1_26245 antibody, ACL antibody, Acly antibody, aclya antibody

背景

Description: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

Aliases: ACL, ATPCL, CLATP

分子量

125kDa

基因ID

47

HGNC

47

UniProt

P53396

途径

Warburg Effect