Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
特异性
CP-226 crossreacts well with CYP1A in liver samples from a wide variety of species, including rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar), Atlantic cod (Gadus morhua), carp (Cyprinus carpio), flounder (Platichthys flesus), sheepshead minnow (Cyprinodon variegatus) and gilthead seabream (Sparus aurata). The degree of cross-reactivity differs between species and with the different methods employed.
免疫原
Immunogen source: Synthetic peptides corresponding to amino acid sequence 190-204 and 282-296 of rainbow trout CYP1A, the latter coupled to the carrier protein keyhole limpet hemocyanin (KLH).
CP-226 works well in both ELISA and western blot for a number of fish species. The antibody also works well in immunohistochemistry. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating. Normal dilution range: ELISA: 1:100 - 1:2000, Western blot: 1:100 - 1:2000
This product consists of affinity-purified rabbit polyclonal antibodies against peptides 190-204 and 282-296 of rainbow trout (Oncorhynchus mykiss) cytochrome P450 1A (CYP1A). The antibodies are dissolved in 0.1 M Na-phosphate, 0.15 M NaCl, 1% BSA, 0.1% Na-azide, pH 7.4.