GM4C1 recognises granzyme B transiently expressed on the cell surface of transfected BOSC cells as well as the native protein in peripheral blood mononuclear cells. The antibody does not cross-react with the human granzymes A, K or M (Fi
纯化方法
Protein G
免疫原
genetic immunisation with cDNA encoding human Granzyme B
GZMB
适用: 人
IHC, ELISA, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Flow cytometry: 1.2 µg/10^6 cells ELISA: 1:200 - 1:400 CELISA: 1:200 - 1:400 For each application a titration should be performed to determine the optimal concentration.
Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. GM4C1 was generated by genetic immunisation and reacts with human granzyme B (GrB), a 27 kDa serine protease which cleaves target-cell proteins at specific aspartate residues.