Uricase (ABC3735) 抗体
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- 抗原
- Uricase (ABC3735)
- 适用
- Bacillus
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- ELISA, Immunoprecipitation (IP), Western Blotting (WB)
- Supplier Product No.
- 200-101-092-0100
- Supplier
- Rockland
- 原理
- Uricase Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].
- 产品特性
- Synonyms: goat anti-uricase antibody, Urate oxidase antibody, Factor-independent urate hydroxylase
- 纯化方法
- Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Uricase [Bacillus species]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Uricase (ABC3735) antibody (Biotin)
ABC3735 适用: Bacillus ELISA, IP, WB 宿主: 山羊 Polyclonal Biotin
anti-Uricase (ABC3735) antibody (HRP)ABC3735 适用: Bacillus ELISA, IP, WB 宿主: 山羊 Polyclonal HRP
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- 应用备注
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Application Note: Uricase Antibody has been tested by western blot and is assayed against 1.0 μg of Uricase [Bacillus species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1,000 - 1:2,500
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Uricase (ABC3735)
- 别名
- ABC3735
- 物质类
- Dinucleotide
- 背景
- Background: Uricase, or urate oxidase, catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. Urate oxidase is found in nearly all organisms, from bacteria to mammals, and plays different metabolic roles, depending on its host organism. Humans are the only animal that are unable to break down uric acid to allantoin. This is because humans do not have the necessary enzyme uricase. Humans do have a gene for urate oxidase, but it is nonfunctional. Thus uric acid is the end product of animal products in humans. This leads to an increased possibility of an accumulation of uric acid in the body when animal products are eaten. Excessive concentration of uric acid in the blood stream leads to gout. It has been proposed that the loss of urate oxidase gene expression has been advantageous to primates, since uric acid is a powerful antioxidant and scavenger of singlet oxygen and radicals. Its presence provides the body with protection from oxidative damage, thus prolonging life and decreasing age-specific cancer rates.
- 基因ID
- 3202700
- NCBI登录号
- YP_177228
- UniProt
- Q5WBJ3
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