p53 抗体 (AA 108-370)
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- 抗原 See all p53 (TP53) 抗体
- p53 (TP53) (Tumor Protein P53 (TP53))
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抗原表位
- AA 108-370
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This p53 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Polyclonal Antibody to Tumor Protein p53 (P53)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.
- 交叉反应
- 大鼠
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Tumor Protein p53 (P53) corresdonding to Gly108~Lys370 with N-terminal His Tag
- 亚型
- IgG
- Top Product
- Discover our top product TP53 Primary Antibody
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- 应用备注
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Western blotting: 0.3-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- 储存液
- ProClin
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes." in: Biochemical and biophysical research communications, Vol. 517, Issue 2, pp. 216-220, (2020) (PubMed).
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Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes." in: Biochemical and biophysical research communications, Vol. 517, Issue 2, pp. 216-220, (2020) (PubMed).
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- 抗原
- p53 (TP53) (Tumor Protein P53 (TP53))
- 别名
- Tumor Protein p53 (TP53 产品)
- 背景
- TP53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
- 途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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