ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
At least three isoforms of SPATA6 are known to exist, this antibody will detect only the longest two isoforms. SPATA6 antibody is predicted to not cross-react with other SPATA protein family members.
纯化方法
Affinity chromatography purified via peptide column
免疫原
SPATA6 antibody was raised against a AA 14 synthetic peptide near the carboxy terminus of human SPATA6.
SPATA6 antibody can be used for detection of SPATA6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Optimal dilutions for each application to be determined by the researcher.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
PBS containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
储存条件
4 °C/-20 °C
储存方法
SPATA6 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year.
Synonyms: Spermatogenesis associated protein 6, HASH, SRF1, SRF-1
The development of spermatogonial stem cells into spermatozoa, i.e., spermatogenesis, is a highly ordered process that requires multiple proteins, including some that are testis-specific or testis-specific isoforms of genes that are expressed in other tissues. One such protein is SPATA6: while its full-length isoform is expressed in multiple tissues including thymus, brain and testis, the shorter isoform is expressed only in testis. It has some homology to the motor domain of kinesin related proteins and with the C. elegans neural calcium sensor protein NCS-2.