(1 reference)-
- 抗原 See all SUMO products
- SUMO
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SUMO antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
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This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO protein.
Immunogentype:Recombinant - 亚型
- IgG
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anti-SUMO antibody
适用: Saccharomyces cerevisiae WB, ELISA, IHC, IP 宿主: 小鼠 Monoclonal 4F2-F5-G2 unconjugated
anti-SUMO antibody适用: Saccharomyces cerevisiae WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-SUMO (AA 37-66) antibody适用: 人, Drosophila melanogaster WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SUMO (pan) antibody适用: 人, 大鼠 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This purified polyclonal antibody reacts with human SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 1:2,000 dilution is recommended. An 11.6 kDa band corresponding to human SUMO is detected. Most human cell lysates can be used as a positive control without induction or stimulation. For ELISA a 1:4,000 to 1:20,000 dilution is recommended. Researchers should determine optimal titers for other applications.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with deionized water (or equivalent)
- 浓度
- 5.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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: "Evidence for intermolecular interactions between the intracellular domains of the arabidopsis receptor-like kinase ACR4, its homologs and the Wox5 transcription factor." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118861, (2016) (PubMed).
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: "Evidence for intermolecular interactions between the intracellular domains of the arabidopsis receptor-like kinase ACR4, its homologs and the Wox5 transcription factor." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118861, (2016) (PubMed).
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- 抗原
- SUMO
- Abstract
- SUMO 产品
- 别名
- CG4494 antibody, Dm0342 antibody, DmSUMO-1 antibody, DmSmt3 antibody, Dmel\\CG4494 antibody, Dmsmt3 antibody, SMT3 antibody, SUMO antibody, Smt3 antibody, Sumo antibody, anon-EST:Posey240 antibody, dSmt3 antibody, dsmt3 antibody, l(2)04493 antibody, l(2)SH0182 antibody, l(2)SH2 0182 antibody, sumo antibody, CG4494 gene product from transcript CG4494-RA antibody, smt3 antibody
- 背景
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Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in
Synonyms: GAP modifying protein 1 antibody, GMP 1 antibody, GMP1 antibody, PIC 1 antibody, PIC1 antibody, SENP2 antibody, Sentrin 1 antibody, Sentrin antibody, Small ubiquitin related modifier 1 antibody - 基因ID
- 7341
- UniProt
- P63165
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