Sucrose Phosphorylase 抗体
-
- 抗原 See all Sucrose Phosphorylase products
- Sucrose Phosphorylase
- 适用
- E. coli
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This Sucrose Phosphorylase antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- Supplier Product No.
- 200-101-231-0100
- Supplier
- Rockland
- 原理
- Sucrose Phosphorylase Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.
- 产品特性
- Synonyms: goat anti-Sucrose Phosphorylase Antibody, Putative sucrose phosphorylase, Sucrose glucosyltransferase
- 纯化方法
- Anti-Sucrose phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
-
Immunogen: Sucrose Phosphorylase [E.coli]
Immunogen Type: Native Protein
- 亚型
- IgG
-
-
- 应用备注
-
Application Note: Anti-Sucrose phosphorylase Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Sucrose Phosphorylase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:6,500 to 1:32,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Sucrose Phosphorylase
- Abstract
- Sucrose Phosphorylase 产品
- 背景
- Background: Sucrose phosphorylase catalyzes as Sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate.
- 基因ID
- 4492194
- NCBI登录号
- YP_852475
- UniProt
- P76041
-
