Rhodamine 抗体
Rockland 200-301-246
ELISA, WB, IHC, DB
宿主: 小鼠
Monoclonal
11H10
unconjugated
2 images
(1 reference)
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- 抗原
- Rhodamine
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)
- Supplier Product No.
- 200-301-246
- Supplier
- Rockland
- 原理
- Rhodamine Antibody
- 交叉反应 （详细）
- Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins.
- 产品特性
- Synonyms: Mouse Anti-Rhodamine Antibody
- 纯化方法
- RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-RHODAMINE Monoclonal Antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.
Immunogen Type: Other
- 克隆位点
- 11H10
- 亚型
- IgG1 kappa
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:10,000
Application Note: This protein A purified monoclonal antibody against rhodamine reacts with most derivative molecules and has been tested for use in ELISA. Optimal concentration in other immunoassays should be determined by the researcher.
Western Blot Dilution: 1:1,000 - 1:10,000
ELISA Dilution: 1:10,000 - 1:30,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "The Src substrate SKAP2 regulates actin assembly by interacting with WAVE2 and cortactin proteins." in: The Journal of biological chemistry, Vol. 288, Issue 2, pp. 1171-83, (2013) (PubMed).
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: "The Src substrate SKAP2 regulates actin assembly by interacting with WAVE2 and cortactin proteins." in: The Journal of biological chemistry, Vol. 288, Issue 2, pp. 1171-83, (2013) (PubMed).
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- 抗原
- Rhodamine
- 物质类
- Chemical
- 背景
- Background: RHODAMINE Monoclonal Antibody specifically detect dyes in the Rhodamine family. Rhodamine is a family member of the fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
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