RFX5 抗体
(3 references)
-
- 抗原 See all RFX5 抗体
- RFX5 (Regulatory Factor X 5 (RFX5))
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This RFX5 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Gel Shift (GS), Chromatin Immunoprecipitation (ChIP)
- Supplier Product No.
- 200-401-194
- Supplier
- Rockland
- 原理
- RFX5 Antibody
- 交叉反应 （详细）
- Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa.
- 产品特性
- Synonyms: rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody
- 纯化方法
- RFXF5 antibody was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product RFX5 Primary Antibody
-
anti-Regulatory Factor X 5 (RFX5) (AA 1-616) antibody
RFX5 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 367-616) antibodyRFX5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 1-616) antibodyRFX5 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 516-616) antibodyRFX5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B8 unconjugated
anti-Regulatory Factor X 5 (RFX5) (Internal Region) antibodyVerified RFX5 适用: 人, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) antibodyRFX5 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (C-Term) antibodyRFX5 适用: 人, 小鼠, 大鼠, 豚鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (N-Term) antibodyRFX5 适用: 人, 犬, 兔, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (N-Term) antibodyRFX5 适用: 人, 犬, 兔 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Gel Shift Dilution: 0.5 μL to 1.0 μL
Application Note: Anti RFX5 antibody has been assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG. Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 μL per assay.
ChIP Dilution: User Optimized
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
-
: "The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma." in: Oncology reports, Vol. 37, Issue 1, pp. 289-296, (2017) (PubMed).
: "In contrast to Chlamydia trachomatis, Waddlia chondrophila grows in human cells without inhibiting apoptosis, fragmenting the Golgi apparatus, or diverting post-Golgi sphingomyelin transport." in: Infection and immunity, Vol. 83, Issue 8, pp. 3268-80, (2015) (PubMed).
: "Regulatory factor for X-box family proteins differentially interact with histone deacetylases to repress collagen alpha2(I) gene (COL1A2) expression." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9260-70, (2006) (PubMed).
-
: "The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma." in: Oncology reports, Vol. 37, Issue 1, pp. 289-296, (2017) (PubMed).
-
- 抗原
- RFX5 (Regulatory Factor X 5 (RFX5))
- 别名
- RFX5 (RFX5 产品)
- 背景
- Background: Anti-RFX5 is specific for the RFX5 protein. RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. RFX5 has been shown to interact with CIITA.
- 基因ID
- 5993
- UniProt
- P48382
-
