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Red Blood Cell 抗体

Rockland 213-4139 RBC 适用: 绵羊 AGG 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN106464
Supplier Product No.: 213-4139
发货至: 中国
  • 抗原 See all Red Blood Cell (RBC) products
    Red Blood Cell (RBC)
    适用
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    绵羊
    宿主
    • 98
    • 17
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    克隆类型
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    多克隆
    标记
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    This Red Blood Cell antibody is un-conjugated
    应用范围
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    Agglutination (AGG)
    Supplier Product No.
    213-4139
    Supplier
    Rockland
    原理
    Sheep Red Blood Cell RBC Antibody
    产品特性
    Synonyms: rabbit Anti-sheep RBC antibody, Sheep Red Blood Cell Antibody, Rabbit anti-sheep Antibody for hemagglutination, rabbit anti-sheep red blood cell, rabbit antibody to sheep Red Blood Cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrocyte, anti-sheep erythrocyte antibody
    纯化方法
    Rabbit Anti-Sheep Red Blood Cell is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Sheep washed pooled Red Blood Cells (RBC)

    Immunogen Type: Other

    亚型
    IgG
  • 应用备注

    Application Note: Rabbit Anti-Sheep RBC Antibody is tested for agglutination of cells on titer plates. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    Other: AGGLUTINATION TITER 1:32 to 1:64

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 5.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Wang, Subramanian, Yurdagul, Barbosa-Lorenzi, Cai, de Juan-Sanz, Ryan, Nomura, Maxfield, Tabas: "Mitochondrial Fission Promotes the Continued Clearance of Apoptotic Cells by Macrophages." in: Cell, Vol. 171, Issue 2, pp. 331-345.e22, (2017) (PubMed).

  • 抗原
    Red Blood Cell (RBC)
    别名
    RBC (RBC 产品)
    背景
    Background: Anti-SHEEP Red Blood Cell Antibody may be used in hemagglutination assays. Anti-SHEEP RBC Antibody is used to sensitize erythrocytes and quantitate agglutination. Hemagglutination assay or HA is a method of quantification for viruses or bacteria by hemagglutination. Some viral families and many bacteria have envelope or surface proteins which are able to agglutinate (stick to) human or animal red blood cells (RBC) and bind to N-acetylneuraminic acid. As each of the agglutinating molecule attaches to multiple RBCs, a lattice-structure will form. Normally, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.1 % to 0.7 % in steps of 0.2 %) for approx. 30 min, often at 4° C, otherwise viruses with neuraminidase activity will detach the virus from the RBCs. Then the lattice forming parts will be counted and the titer calculated. The titer of a hemagglutination assay is determined by the last viable "lattice" structure found. This is because it is at the point where, if diluted anymore, the amount of Virus particles will be less than that of the RBCs and thus not be able to agglutinate them together.
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