(1 reference)
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- 抗原 See all Red Blood Cell (RBC) products
- Red Blood Cell (RBC)
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Red Blood Cell antibody is un-conjugated
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应用范围
- Agglutination (AGG), Immunohistochemistry (IHC)
- Supplier Product No.
- 209-4139
- Supplier
- Rockland
- 原理
- Human Red Blood Cell Antibody
- 产品特性
- Synonyms: rabbit Anti-human RBC antibody, Red blood cell antibody, Antibody for hemagglutination, rabbit anti-human red blood cell, rabbit antibody to human red blood cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrocytes, anti-erythrocytes antibody
- 纯化方法
- This product is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
- Optional[Immunogen]: Human washed pooled Red Blood Cells (RBC)
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry Dilution: 2.5 μg/mL
Application Note: Anti-Human Red Blood Cells Antibody has been tested by agglutination and immunohistochemistry. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers. Specific conditions should be optimized by researcher.
Other: AGGLUTINATION TITER 1:32 - 1:64
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 5.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "NK cells inhibit Plasmodium falciparum growth in red blood cells via antibody-dependent cellular cytotoxicity." in: eLife, Vol. 7, (2019) (PubMed).
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: "NK cells inhibit Plasmodium falciparum growth in red blood cells via antibody-dependent cellular cytotoxicity." in: eLife, Vol. 7, (2019) (PubMed).
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- 抗原
- Red Blood Cell (RBC)
- 别名
- RBC (RBC 产品)
- 背景
- Background: Anti-Human Red Blood Cells Antibody recognizes human red blood cells and can be used in a variety of agglutination assays where agglutination or clumping of red blood cells is a positive indication of the presence of an analyte, virus particle or bacteria. Red blood cells (RBCs), also known as erythrocytes, are metabolically active cells that are highly adapted to serve their function in blood gas exchange (oxygen/CO2 transport). The red blood cells enable the transport of sufficient O2 between respiratory surfaces (lungs, gills) and metabolizing tissues by means of their high intracellular concentration of hemoglobin.
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