RAD9A 抗体 (pSer1260)
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- 抗原 See all RAD9A 抗体
- RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))
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抗原表位
- pSer1260
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RAD9A antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 600-401-468
- Supplier
- Rockland
- 原理
- Rad9 phospho S1260 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against the phosphorylated form of yeast Rad9 at the pS1260 residue.
- 产品特性
- Synonyms: Rabbit anti-Rad9 pS1260 antibody, Rabbit anti-Rad9 phospho antibody, Rad 9, Rad-9, Cell cycle checkpoint control protein antibody, Cell cycle checkpoint control protein RAD9A antibody, DNA repair exonuclease rad9 homolog A antibody, DNA repair protein RAD9, YDR217C, YD9934.02C
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to phosphorylated form of an internal region near aa 1225-1275 from the aa1309 yeast Rad9 protein conjugated to KLH.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product RAD9A Primary Antibody
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anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 162-391) antibody
RAD9A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (Center) antibodyRAD9A 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) antibodyRAD9A 适用: 人, 猴, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D9 unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (pSer328) antibodyRAD9A 适用: 人 WB, IHC (p), DB 宿主: 兔 Polyclonal RB7115 unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 267-341) antibodyRAD9A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 29-62), (N-Term) antibodyRAD9A 适用: 小鼠 WB 宿主: 兔 Polyclonal RB52362 unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 279-313), (C-Term) antibodyRAD9A 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB51320 unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) antibodyRAD9A 适用: 人, 小鼠 WB, IP 宿主: 小鼠 Monoclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (C-Term) antibodyRAD9A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (C-Term) antibodyVerified RAD9A 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Application Note: This phospho specific polyclonal antibody was tested by immunoblotting and ELISA. Data from both immunoblotting and ELISA indicate the antibody is reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting detects yeast Rad9 protein. No reactivity is expected against the human or mouse analogs of RAD9. Reactivity against RAD9 from other sources is unknown. Cross reactivity may occur with auto-phosphorylated Rad53 kinase. Although not tested, this antibody is likely functional by IHC and IP. This product has been assayed against 0.1 μg of phosphorylated peptide (pS1260) in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) code # TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 is suggested for this product. Minimal reactivity was detected against the non-phosphorylated form (S1260) of the immunizing peptide. This antibody appears to be specific for the active form (phosphorylated) of the protein. Dilute the antibody 1:100 to 1:500 for immunoblotting. Researchers should determine optimal titers for other applications.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.41 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))
- 别名
- RAD9 (RAD9A 产品)
- 背景
- Background: Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1. DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9. Cells have evolved multiple strategies for tolerating genomic damage. The most important of these are numerous repair systems that remove or bypass potentially mutagenic DNA lesions. Another cellular strategy is to delay cell-cycle transitions at multiple points. The genetic control of these delays, termed `checkpoints', was first established in budding yeast where it was shown that the RAD9 gene functions in G2/M arrest after irradiation with X-rays. Subsequently, it has become clear that Rad9 also functions at the G1/S, intra-S and mid-anaphase checkpoints. Defects in checkpoint regulation can lead to genome instability and, in higher eukaryotes, neoplastic transformation. Rad9 also controls the transcriptional induction of a DNA damage regulon (DDR). Rad9 may also have a pro-apoptotic function. This is suggested in that Rad9 from Schizosaccharomyces pombe (SpRad9) contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells. Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p, is hyperphos-phorylated by Mec1p and Tel1p, and is a potential Cdc28p substrate. Mature yeast Rad9 is reported to have an apparent molecular weight of ~148 kDa. The human homolog is reported at 48.5 kDa.
- 基因ID
- 851803
- NCBI登录号
- NP_010503
- UniProt
- P14737
- 途径
- Positive Regulation of Response to DNA Damage Stimulus
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