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PKM 抗体

Rockland 100-1178 PKM 适用: 兔 ELISA, WB, IHC, IP 宿主: 山羊 Polyclonal unconjugated
Rockland
产品编号 ABIN106387
Supplier Product No.: 100-1178
发货至: 中国
  • 抗原 See all PKM 抗体
    PKM (Pyruvate Kinase M1/2 (PKM))
    适用
    • 39
    • 11
    • 5
    • 2
    宿主
    • 36
    • 5
    • 2
    山羊
    克隆类型
    • 37
    • 6
    多克隆
    标记
    • 25
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PKM antibody is un-conjugated
    应用范围
    • 28
    • 22
    • 13
    • 9
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Supplier Product No.
    100-1178
    Supplier
    Rockland
    原理
    Pyruvate Kinase Antibody
    交叉反应 (详细)
    This product has been reported to react with all forms of pyruvate kinase (pan M-PK).
    产品特性
    Synonyms: goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2
    纯化方法
    Anti-PYRUVATE KINASE Antibody immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Pyruvate Kinase [Rabbit Muscle].
    免疫原

    Immunogen: Pyruvate Kinase [Rabbit Muscle]

    Immunogen Type: Native Protein

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  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-PYRUVATE KINASE Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Specific conditions should be optimized by user.

    Western Blot Dilution: 1:5,000 - 1:20,000

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:50,000 - 1:200,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    40 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Shimada, Shinagawa, Ishii: "Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 3, pp. 245-54, (2008) (PubMed).

  • 抗原
    PKM (Pyruvate Kinase M1/2 (PKM))
    别名
    PKM (PKM 产品)
    背景
    Background: Pyruvate kinase (EC: 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a magnesium ion. The enzyme is a 'transferase' under the international classification of enzymes. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. However, in anaerobic glycolysis, lactate dehydrogenase will utilize the NADH produced by glyceraldehyde phosphate dehydrogenase to reduce pyruvate to lactate. In humans, there are two pyruvate kinase isozymes: type M (muscle, SwissProt P14618) and type L,R (liver and erythrocyte, SwissProt P30613). The isozymes differ in primary structure and regulation. This reaction has a large positive free energy change, one of three in glycolysis. All three such steps regulate the overall activity of the pathway, and are, in general, irreversible under wild-type conditions. Anti-Pyruvate kinase antibody is idea for investigators involved in cytokines and growth factor research.
    基因ID
    100008676
    NCBI登录号
    NP_001182574
    UniProt
    P11974
    途径
    Warburg Effect
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