PPARA 抗体 (N-Term)
(3 references)-
- 抗原 See all PPARA 抗体
- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
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抗原表位
- N-Term
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适用
- 小鼠, 大鼠, 犬, Cow, Pig, Golden Syrian Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PPARA antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- This affinity purified antibody is directed against mouse PPAR alpha protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
- 产品特性
- Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
- 纯化方法
- affinity purified
- 过滤
- Sterile filtered
- 免疫原
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This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1 to 18 of mouse PPAR alpha.
Immunogen Type: Peptide - 亚型
- IgG
- Top Product
- Discover our top product PPARA Primary Antibody
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anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 301-400) antibody
PPARA 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 61-170) antibodyPPARA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 1-120) antibodyPPARA 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5D10C3 unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 1-468) antibodyPPARA 适用: 人, 小鼠 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal 1331CT894-186-143 unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (N-Term) antibodyKO Validated PPARA 适用: 人 WB, IP, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 1-120) antibodyPPARA 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5D10E10 unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (C-Term) antibodyPPARA 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, Pig, 豚鼠, 山羊, 兔, 绵羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (pSer12) antibodyPPARA 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (N-Term) antibodyPPARA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (N-Term) antibodyPPARA 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠, 山羊, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a single band approximately 52 kDa in size corresponding to PPAR alpha by western blot in the appropriate tissue or cell lysate. A 1:200 dilution is suggested for IHC. Specific conditions for reactivity should be optimized by the end user.
- 说明
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Gene Name: PPARA
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "Fenofibrate induces effective apoptosis in mantle cell lymphoma by inhibiting the TNFalpha/NF-kappaB signaling axis." in: Leukemia, Vol. 24, Issue 8, pp. 1476-86, (2010) (PubMed).
: "Medicine. PPARs as therapeutic targets: reverse cardiology?" in: Science (New York, N.Y.), Vol. 302, Issue 5644, pp. 406-7, (2003) (PubMed).
: "Human and rat peroxisome proliferator activated receptors (PPARs) demonstrate similar tissue distribution but different responsiveness to PPAR activators." in: The Journal of steroid biochemistry and molecular biology, Vol. 51, Issue 3-4, pp. 157-66, (1995) (PubMed).
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: "Fenofibrate induces effective apoptosis in mantle cell lymphoma by inhibiting the TNFalpha/NF-kappaB signaling axis." in: Leukemia, Vol. 24, Issue 8, pp. 1476-86, (2010) (PubMed).
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- 抗原
- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
- 别名
- PPAR alpha (PPARA 产品)
- 别名
- NR1C1 antibody, PPAR antibody, PPARalpha antibody, hPPAR antibody, LOC100136415 antibody, PPARALPHA antibody, nr1c1 antibody, xPPARalpha antibody, xppara antibody, 4933429D07Rik antibody, AW742785 antibody, Nr1c1 antibody, PPAR-alpha antibody, Ppar antibody, peroxisome proliferator activated receptor alpha antibody, peroxisome proliferator-activated receptor alpha antibody, peroxisome proliferator activated receptor alpha L homeolog antibody, PPARA antibody, LOC100136415 antibody, ppara.L antibody, Ppara antibody, ppara antibody
- 背景
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Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
Synonyms: hPPAR antibody, MGC2237 antibody, MGC2452 antibody, NR1C1 antibody, Nuclear receptor subfamily 1 group C member 1 antibody, Peroxisome Proliferator Activated Receptor alpha antibody, PPAR antibody - 基因ID
- 19013, 31543500
- UniProt
- P23204
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
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