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Beta-Phosphoglucomutase 抗体

Rockland 200-101-237-0100 适用: Lactococcus lacti WB, ELISA 宿主: 山羊 Polyclonal unconjugated
Rockland
产品编号 ABIN105570
Supplier Product No.: 200-101-237-0100
发货至: 中国
  • 抗原 See all Beta-Phosphoglucomutase products
    Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)
    适用
    • 4
    • 3
    Lactococcus lacti
    宿主
    • 4
    • 3
    山羊
    克隆类型
    • 7
    多克隆
    标记
    • 4
    • 2
    • 1
    This Beta-Phosphoglucomutase antibody is un-conjugated
    应用范围
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    200-101-237-0100
    Supplier
    Rockland
    原理
    Beta-Phosphoglucomutase Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified b-Phosphoglucomutase [Lactococcus lacti].
    产品特性
    Synonyms: goat anti-Beta-Phosphoglucomutase Antibody, goat anti-Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody
    纯化方法
    Anti-Beta-Phosphoglucomutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Beta Phosphoglucomutase [Lactococcus lacti]

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Application Note: Anti-Beta-Phosphoglucomutase Antibody has been tested by ELISA and western blot. This antibody is assayed against 1.0 μg of b-Phosphoglucomutase [Lactococcus lacti] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)
    别名
    pgmB (Beta-Phosphoglucomutase 产品)
    背景
    Background: Beta-Phosphoglucomutase catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C1 anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose. The catalysis proceeds via a phosphoenzyme formed by reaction of an active-site nucleophile with the cofactor glucose 1,6-diphosphate (G1,6-diP). The phosphorylated mutase binds either G1P or G6P and transfers the phosphoryl group to the C6OH or C1OH, respectively.
    基因ID
    1114041
    UniProt
    P71447
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