Western Blotting (WB), Immunoprecipitation (IP), ELISA
交叉反应 (详细)
Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.
纯度
Anti-PENICILLINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Penicillinase [E.cloacae].
内毒素水平
Low Endotoxin : No
免疫原
Penicillinase [E.cloacae] Immunogen Type: Native Protein
适用: E. coli
WB, EIA
宿主: 小鼠
Monoclonal
8A5-A10
unconjugated
应用备注
PENICILLINASE ANTIBODY has been assayed against 1.0 µg of Penicillinase [E.cloacae] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7.000 to 1:21.000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5.000 - 1:20.000 IF Immunoprecipitation Dilution: 1:100 Western Blot Dilution: 1:500 - 1:5.000
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
注意事项
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
储存条件
-20 °C
储存方法
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
有效期
Expiration date is one (1) year from date of opening.