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PAK1/2/3 抗体 (pThr423)

PAK1/2/3 适用: 人 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN105217
发货至: 中国
  • 抗原 See all PAK1/2/3 抗体
    PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))
    抗原表位
    • 11
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    • 1
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    pThr423
    适用
    • 32
    • 27
    • 26
    • 1
    • 1
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    宿主
    • 33
    克隆类型
    • 32
    • 1
    多克隆
    标记
    • 26
    • 1
    • 1
    • 1
    • 1
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    This PAK1/2/3 antibody is un-conjugated
    应用范围
    • 19
    • 18
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    • 6
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    • 2
    • 1
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    Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    免疫原
    Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
    亚型
    IgG
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    Discover our top product PAK1/2/3 Primary Antibody
  • 应用备注
    This phospho specific polyclonal antibody was tested by ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.   Although not tested, this antibody is likely functional in immunohistochemistry, immunoblotting, and immuno-precipitation.  Lysates from Jurkat cells or PAK transfected cells may be used as a control.This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.94 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • 抗原
    PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))
    别名
    PAK 1/2/3 (PAK1/2/3 产品)
    别名
    X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, pak1.S antibody, PAK1 antibody
    背景
    The p21-activated kinases (PAKs) are a family of multifunctional serine/threonine kinases involved in a variety of cell functions including stress response, apoptosis and regulation of cell motility and tumor metastasis.  Mammalian PAKs are called 1, 2, 3 or a, g, b respectively.  PAKs are part of a large family of kinases where the catalytic domain of the kinase is related to Ste20 kinase of S. cerevisiae.  Pak activity is regulated by specific binding of GTP-bound Rac and cdc42 GTPases and also by sphingosine and related lipids.  PAK1 activation is induced by a variety of growth factors and G-protein-coupled receptors, Fc receptors, and integrins.  This antibody is specific for the phosphorylated form of PAK 1/2/3.  The selected peptide sequence used to generate the polyclonal antibody is common to all human PAKs.
    Synonyms: Alpha PAK antibody, Gamma PAK antibody, Beta PAK antibody
    基因ID
    5058
    UniProt
    Q13153
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