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- 抗原 See all PAK1/2/3 抗体
- PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))
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抗原表位
- pThr423
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PAK1/2/3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
- Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- 亚型
- IgG
- Top Product
- Discover our top product PAK1/2/3 Primary Antibody
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anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (Thr402), (Thr421), (Thr423) antibody
PAK1/2/3 适用: 人 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (Thr402), (Thr421), (Thr423) antibodyPAK1/2/3 适用: 人, 大鼠, 小鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (pThr423) antibodyPAK1/2/3 适用: 人, 大鼠, 小鼠, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (AA 391-440) antibodyPAK1/2/3 适用: 人, 大鼠, 小鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (AA 111-160), (pSer144) antibodyPAK1/2/3 适用: 人, 大鼠, 小鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This phospho specific polyclonal antibody was tested by ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry, immunoblotting, and immuno-precipitation. Lysates from Jurkat cells or PAK transfected cells may be used as a control.This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 is suggested for this product. Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide. Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 is suggested for this product. Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide. Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 is suggested for this product. Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide. Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 is suggested for this product. Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide. Researchers should determine optimal titers for other applications.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.94 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))
- 别名
- PAK 1/2/3 (PAK1/2/3 产品)
- 别名
- X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, pak1.S antibody, PAK1 antibody
- 背景
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The p21-activated kinases (PAKs) are a family of multifunctional serine/threonine kinases involved in a variety of cell functions including stress response, apoptosis and regulation of cell motility and tumor metastasis. Mammalian PAKs are called 1, 2, 3 or a, g, b respectively. PAKs are part of a large family of kinases where the catalytic domain of the kinase is related to Ste20 kinase of S. cerevisiae. Pak activity is regulated by specific binding of GTP-bound Rac and cdc42 GTPases and also by sphingosine and related lipids. PAK1 activation is induced by a variety of growth factors and G-protein-coupled receptors, Fc receptors, and integrins. This antibody is specific for the phosphorylated form of PAK 1/2/3. The selected peptide sequence used to generate the polyclonal antibody is common to all human PAKs.
Synonyms: Alpha PAK antibody, Gamma PAK antibody, Beta PAK antibody - 基因ID
- 5058
- UniProt
- Q13153
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