p21 抗体
(6 references)
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- 抗原 See all p21 (CDKN1A) 抗体
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This p21 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Multiplex Assay (MA), Fluorescence Microscopy (FM)
- Supplier Product No.
- 200-301-171
- Supplier
- Rockland
- 原理
- p21 WAF1 Antibody
- 交叉反应 （详细）
- This protein A purified mouse monoclonal antibody reacts with human and mammalian p21. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1).
- 产品特性
- Synonyms: mouse anti-p21/WAF1 antibody, Cyclin-dependent kinase inhibitor 1A (p21, CIP1) antibody, DNA Synthesis Inhibitor antibody, MDA 6 antibody, MDA6 antibody, Melanoma Differentiation Associated Protein 6 antibody
- 纯化方法
- Protein A purified
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.
Immunogen Type: Recombinant Protein
- 克隆位点
- WA-1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CDKN1A Primary Antibody
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anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 43-142) antibody
CDKN1A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 65-164) antibodyCDKN1A 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2F1 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibodyKO Validated CDKN1A 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-164) antibodyCDKN1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-100) antibodyCDKN1A 适用: Synthetic WB, IHC (f) 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibodyCDKN1A 适用: 人, Non-Human Primate ELISA, IHC (p), ICC 宿主: 小鼠 Monoclonal WA-1 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 21-100) antibodyCDKN1A 适用: 人, 小鼠, 大鼠, 绵羊 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibodyCDKN1A 适用: 人 WB, IF, IHC, IHC (f) 宿主: 小鼠 Monoclonal CIP1-823 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibodyCDKN1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibodyCDKN1A 适用: 人 WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-p21/WAF1 has been tested in ELISA, western blot, and immunofluorescence. This product is also suitable for immunohistochemistry. The antibody detects a 21 kDa band corresponding to the p21 protein. MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry. Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining). In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein. This antibody immunoprecipitates monomeric p21WAF1 and not p21 complexes with other proteins. High level expression of p21WAF1 requires induction of wild-type p53 expression (e.g. via exposure to DNA damaging agents).
Western Blot Dilution: 1:2,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
IF Microscopy Dilution: 1:250
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Nutlin-3 radiosensitizes hypoxic prostate cancer cells independent of p53." in: Molecular cancer therapeutics, Vol. 7, Issue 4, pp. 993-9, (2008) (PubMed).
: "Inhibition of tumor cell growth by p21WAF1 adenoviral gene transfer in lung cancer." in: Cancer gene therapy, Vol. 5, Issue 3, pp. 183-91, (1998) (PubMed).
: "p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells." in: Oncogene, Vol. 15, Issue 16, pp. 1903-9, (1997) (PubMed).
: "The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo." in: Nature medicine, Vol. 1, Issue 10, pp. 1052-6, (1996) (PubMed).
: "p21 is necessary for the p53-mediated G1 arrest in human cancer cells." in: Cancer research, Vol. 55, Issue 22, pp. 5187-90, (1995) (PubMed).
: "A cis-acting element in Rous sarcoma virus long terminal repeat required for promoter repression by HeLa nuclear protein p21." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15815-20, (1995) (PubMed).
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: "Nutlin-3 radiosensitizes hypoxic prostate cancer cells independent of p53." in: Molecular cancer therapeutics, Vol. 7, Issue 4, pp. 993-9, (2008) (PubMed).
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- 抗原
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- 别名
- CDKN1A (CDKN1A 产品)
- 背景
- Background: p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA. Anti-p21WAF1 antibody is useful for researches interested in studying tongue and gastrointestinal cancers, as well as, ubiquitin and cyclin binding proteins.
- 基因ID
- 1026
- NCBI登录号
- NP_510867
- UniProt
- P38936
- 途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
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