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Ovalbumin 抗体

Rockland 200-401-033 OVA 适用: 小鸡 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN105182
Supplier Product No.: 200-401-033
发货至: 中国
  • 抗原 See all Ovalbumin (OVA) 抗体
    Ovalbumin (OVA)
    适用
    • 40
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    小鸡
    宿主
    • 38
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    克隆类型
    • 38
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    多克隆
    标记
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    This Ovalbumin antibody is un-conjugated
    应用范围
    • 37
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    • 5
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
    Supplier Product No.
    200-401-033
    Supplier
    Rockland
    原理
    Ovalbumin Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].
    产品特性
    Synonyms: rabbit anti-Ovalbumin Antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White
    纯化方法
    Rabbit Anti-Ovalbumin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Ovalbumin [Hen Egg White]

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product OVA Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:300 - 1:2,000

    Application Note: Anti-Ovalbumin Antibody has been tested in Western Blot. Although not tested this product could be useful in assays such as ELISA, IHC and IP. Specific conditions for reactivity and signal detection should be optimized by the end user.

    Western Blot Dilution: 1:500 - 1:2,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Apperson, Vorachek, Dolan, Bobe, Pirelli, Hall: "Effects of feeding pregnant beef cows selenium-enriched alfalfa hay on passive transfer of ovalbumin in their newborn calves." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 50, pp. 640-645, (2019) (PubMed).

  • 抗原
    Ovalbumin (OVA)
    别名
    SERPINB14 (OVA 产品)
    背景
    Background: Anti-Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 55-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins, it does not undergo a conformational change upon proteolytic cleavage and is not a serine protease inhibitor. The function of ovalbumin is still unknown, although it is presumed to be a storage protein for tissue-specific, steroid hormone-induced gene expression. Ovalbumin is a key protein for biochemical investigations and processes and can be used as a control, blocking reagent, or antibody in assays such as ELISA, SDS-PAGE, IHC, Western Blot, and size exclusion chromatography.
    基因ID
    396058
    UniProt
    P01012
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