C3 抗体 (AA 1580-1592)
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- 抗原 See all C3 抗体
- C3 (Complement Component 3 (C3))
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抗原表位
- AA 1580-1592
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This C3 antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (IHC)
- 原理
- complement C3 (aa1580-1592)
- 序列
- QQRTFISPIK CRE
- 特异性
- This antibody is expected to recognize the C3 alpha chain, C3b alpha' chain and the C3c alpha' chain fragment 2.
- 预测反应
- Human
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence QQRTFISPIKCRE, from the C Terminus of the protein sequence according to NP_000055.2.
- 亚型
- IgG
- Top Product
- Discover our top product C3 Primary Antibody
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- 应用备注
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DS WB Results: Preliminary experiments gave an approx 125 kDa band in Human Liver lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104 kDa according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125 kDa band was successfully blocked by incubation with the immunizing peptide.
DS IHC Results: Paraffin embedded Human Placenta and Testis. Recommended concentration: 5 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:128000.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- C3 (Complement Component 3 (C3))
- 别名
- Complement Component 3 (C3 产品)
- 背景
- C3, complement component 3, AHUS5, ARMD9, ASP, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, OTTHUMP00000197086, acylation-stimulating protein cleavage product, complement C3, complement component C3
- 基因ID
- 718
- NCBI登录号
- NP_000055
- 途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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