NCKAP1 抗体 (Internal Region)
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- 抗原 See all NCKAP1 抗体
- NCKAP1 (NCK Associated Protein 1 (NCKAP1))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This NCKAP1 antibody is un-conjugated
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应用范围
- ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- NCKAP1
- 序列
- TPREYLTSHL EIR
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_038464.1, NP_995314.1).
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-TPREYLTSHLEIR, from the of the protein sequence according to NP_038464.1, NP_995314.1.
- 亚型
- IgG
- Top Product
- Discover our top product NCKAP1 Primary Antibody
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anti-NCK Associated Protein 1 (NCKAP1) antibody
NCKAP1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NCK Associated Protein 1 (NCKAP1) antibodyNCKAP1 适用: 人 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-NCK Associated Protein 1 (NCKAP1) antibodyNCKAP1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-NCK Associated Protein 1 (NCKAP1) (AA 1-250) antibodyNCKAP1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: Preliminary experiments gave an approx 100 kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5 μg/mL antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calcu
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- NCKAP1 (NCK Associated Protein 1 (NCKAP1))
- 别名
- NCKAP1 (NCKAP1 产品)
- 别名
- HEM2 antibody, NAP1 antibody, NAP125 antibody, p125Nap1 antibody, C79304 antibody, H19 antibody, Hem-2 antibody, Hem2 antibody, Nap1 antibody, mKIAA0587 antibody, mh19 antibody, NCK associated protein 1 antibody, NCK-associated protein 1 antibody, NCK-associated protein 1 L homeolog antibody, NCKAP1 antibody, Nckap1 antibody, nckap1.L antibody
- 背景
- NCKAP1, NCK-associated protein 1, FLJ11291, HEM2, KIAA0587, MGC8981, NAP1, NAP125, NAP 1, OTTHUMP00000163460, OTTHUMP00000163461, membrane-associated protein HEM-2, nck-associated protein 1, p125Nap1
- 基因ID
- 10787, 50884, 58823
- NCBI登录号
- NP_038464, NP_995314
- 途径
- RTK signaling, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
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