VIPR1 抗体 (Cytoplasmic Domain)
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- 抗原 See all VIPR1 抗体
- VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))
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抗原表位
- Cytoplasmic Domain
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VIPR1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human VIP Receptor 1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GHRHR (56 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Monkey, Marmoset, Rabbit (94%) Hamster, Pig (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human VIP Receptor 1. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Monkey, Marmoset, Rabbit (94%), Hamster, Pig (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product VIPR1 Primary Antibody
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anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) (AA 351-457) antibody
VIPR1 适用: 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) (AA 52-65), (Extracellular), (N-Term) antibodyVIPR1 适用: 人, 大鼠, 小鼠 WB, IHC, IF, FACS, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) (AA 305-334), (C-Term) antibodyVIPR1 适用: 人 WB 宿主: 兔 Polyclonal RB40946 unconjugated
anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) (Cytoplasmic Domain) antibodyVIPR1 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) (Internal Region) antibodyVIPR1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) (Internal Region) antibodyVIPR1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) antibodyVIPR1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) (AA 1-457) antibodyVIPR1 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) (AA 1-457) antibodyVIPR1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Vasoactive Intestinal Peptide Receptor 1 (VIPR1) (Internal Region) antibodyVerified VIPR1 适用: 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))
- 别名
- VIPR1 (VIPR1 产品)
- 别名
- HVR1 antibody, II antibody, PACAP-R-2 antibody, PACAP-R2 antibody, RDC1 antibody, V1RG antibody, VAPC1 antibody, VIP-R-1 antibody, VIPR antibody, VIRG antibody, VPAC1 antibody, VPAC1R antibody, VPCAP1R antibody, CVIPR antibody, AV071699 antibody, VIP-R1 antibody, RATVASREC antibody, VASREC antibody, VIPR1 antibody, LOC100226500 antibody, vipr1 antibody, hvr1 antibody, rdc1 antibody, v1rg antibody, vipr antibody, virg antibody, vapc1 antibody, vpac1 antibody, vpcap1 antibody, vpac1-r antibody, VIP-R antibody, vasoactive intestinal peptide receptor 1 antibody, vasoactive intestinal peptide receptor 1b antibody, vasoactive intestinal peptide receptor 1 S homeolog antibody, vasoactive intestinal polypeptide receptor antibody, VIPR1 antibody, Vipr1 antibody, vipr1b antibody, vipr1 antibody, vipr1.S antibody, LOC100303683 antibody
- 背景
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Name/Gene ID: VIPR1
Subfamily: Vasoactive intestinal polypeptide
Family: GPCR
Synonyms: VIPR1, HVR1, PACAP type II receptor, PACAP-R-2, Pvr2, RDC1, Vip receptor subtype 1, Vpac1 receptor, V1RG, VIP receptor 1, VIPR, VIRG, VPCAP1R, PACAP-R2, VAPC1, VIP and PACAP receptor 1, VPAC1R, Pacap receptor, type ii, Type II PACAP receptor, VIP receptor, type I, VIP-R-1, VIP1 receptor, VPAC1 - 基因ID
- 7433
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