TM9SF3 抗体 (Internal Region)
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- 抗原 See all TM9SF3 products
- TM9SF3 (Transmembrane 9 Superfamily Member 3 (TM9SF3))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, Cow, 马, 大鼠, 兔, 犬, 豚鼠, 小鸡, Pig, 猴, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TM9SF3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human TM9SF3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%) Lizard, Stickleback, Medaka (93%) Bat, Salmon, Zebrafish, Eye worm, Tick (87%) Pufferfish, Drosophila, Water flea, Nematode (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from internal region of human TM9SF3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Lizard, Stickleback, Medaka (93%), Bat, Salmon, Zebrafish, Eye worm, Tick (87%), Pufferfish, Drosophila, Water flea, Nematode (80%).
Type of Immunogen: Synthetic peptide
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anti-Transmembrane 9 Superfamily Member 3 (TM9SF3) (AA 133-300) antibody
TM9SF3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane 9 Superfamily Member 3 (TM9SF3) (AA 187-236) antibodyTM9SF3 适用: 人, 小鼠, Cow, 马, 大鼠, 兔, 豚鼠, 小鸡, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane 9 Superfamily Member 3 (TM9SF3) (Internal Region), (N-Term) antibodyTM9SF3 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane 9 Superfamily Member 3 (TM9SF3) (N-Term) antibodyTM9SF3 适用: 人, 小鼠, Cow, 马, 大鼠, 兔, 犬, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- TM9SF3 (Transmembrane 9 Superfamily Member 3 (TM9SF3))
- 别名
- TM9SF3 (TM9SF3 产品)
- 别名
- fb75h12 antibody, zgc:56246 antibody, wu:fa94f05 antibody, wu:fb75h12 antibody, SMBP antibody, EP70-P-iso antibody, 1810073M23Rik antibody, 2810031D16Rik antibody, AI115521 antibody, AI413748 antibody, AW146116 antibody, AW549777 antibody, Smbp antibody, mKIAA4036 antibody, RGD1564625 antibody, transmembrane 9 superfamily member 3 antibody, Transmembrane 9 superfamily member 3 antibody, TM9SF3 antibody, tm9sf3 antibody, LOC551549 antibody, Tsp_02755 antibody, tm9s3 antibody, Tm9sf3 antibody
- 背景
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Name/Gene ID: TM9SF3
Synonyms: TM9SF3, EP70-P-iso, RP11-34E5.1, SMBP, SM-11044 binding protein, SM-11044-binding protein - 基因ID
- 56889
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