STX1A 抗体 (N-Term)
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- 抗原 See all STX1A 抗体
- STX1A (Syntaxin 1A (Brain) (STX1A))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠, 猴, Pig, Hamster, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This STX1A antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human STX1A / Syntaxin 1A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except STX2 (67 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%) Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%) Elephant (87%) Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from N-Terminus of human STX1A / Syntaxin 1A. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%), Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%), Elephant (87%), Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product STX1A Primary Antibody
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anti-Syntaxin 1A (Brain) (STX1A) (AA 1-251) antibody
STX1A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11-1A8 unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-255) antibodySTX1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) antibodySTX1A 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-251) antibodySTX1A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 1-251) antibodySTX1A 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) antibodySTX1A 适用: 人, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (N-Term) antibodySTX1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (pSer14) antibodySTX1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) (AA 2-228) antibodySTX1A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 1A (Brain) (STX1A) antibodySTX1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- STX1A (Syntaxin 1A (Brain) (STX1A))
- 别名
- STX1A / Syntaxin 1A (STX1A 产品)
- 别名
- HPC-1 antibody, P35-1 antibody, STX1 antibody, SYN1A antibody, STX1A antibody, hpc-1 antibody, p35-1 antibody, stx1 antibody, sx1a antibody, syntaxin1a antibody, CG10716 antibody, CG18615 antibody, CG31136 antibody, CG5448 antibody, CT30033 antibody, Dm Syx1 antibody, DmSyx1A antibody, Dmel\\CG31136 antibody, SYX1A antibody, Syt1A antibody, Syx antibody, Syx-1A antibody, Syx1 antibody, Syx1a antibody, SyxA antibody, anon-EST:Gibbs4 antibody, anon-WO02059370.54 antibody, dSyx1 antibody, l(3)06737 antibody, synt antibody, syt-1A antibody, syx antibody, syx-1 antibody, syx-1A antibody, syx1 antibody, syx1A antibody, syntaxin 1A antibody, syntaxin 1A (brain) antibody, Syntaxin 1A antibody, syntaxin-1A antibody, STX1A antibody, Stx1a antibody, stx1a antibody, Syx1A antibody, EDI_005240 antibody, Tsp_07354 antibody
- 背景
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Name/Gene ID: STX1A
Family: Syntaxin/epimorphin
Synonyms: STX1A, Syntaxin-1A, p35-1, SYN1a, Syntaxin 1A, Syntaxin 1A (brain), HPC-1, Neuron-specific antigen HPC-1, STX1 - 基因ID
- 6804
- 途径
- Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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