SMO 抗体 (N-Term)
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- 抗原 See all SMO 抗体
- SMO (Smoothened Homolog (Drosophila) (SMO))
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抗原表位
- N-Term
- 适用
- 人, 小鼠, 大鼠, 犬, 猴, Pig, 兔, Cow, Hamster, 马, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SMO antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 品牌
- IHC-plus™
- 特异性
- Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from N-terminal extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%), Opossum (93%), Turkey, Chicken, Xenopus (87%), Zebrafish, Seq squirt (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SMO Primary Antibody
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anti-Smoothened Homolog (Drosophila) (SMO) (AA 551-650) antibody
SMO 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (AA 554-787) antibodySMO 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (AA 28-233) antibodySMO 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (AA 653-787) antibodySMO 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D9 unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (AA 68-117) antibodySMO 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (1st Extracellular Domain) antibodySMO 适用: 人, 小鼠 IHC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibodySMO 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) antibodySMO 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3D10 unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibodySMO 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (AA 56-155) antibodySMO 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3G8 unconjugated
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- 应用备注
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Approved: ICC, IF, IHC, IHC-P (10 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. - 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- SMO (Smoothened Homolog (Drosophila) (SMO))
- 别名
- SMO / Smoothened (SMO 产品)
- 别名
- FZD11 antibody, Gx antibody, SMOH antibody, Smoh antibody, E130215L21Rik antibody, bnb antibody, smoothened antibody, XSmo antibody, smoothened, frizzled class receptor antibody, smoothened, frizzled class receptor L homeolog antibody, SMO antibody, Smo antibody, smo.L antibody, smo antibody
- 背景
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Name/Gene ID: SMO
Subfamily: Frizzled
Family: GPCR
Synonyms: SMO, Frizzled family member 11, FZD11, Gx, Protein Gx, SMOH, Smoothened, Smoothened homolog - 基因ID
- 6608
- 途径
- Hedgehog Signaling
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