S1PR5 抗体 (N-Term)
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- 抗原 See all S1PR5 抗体
- S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))
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抗原表位
- N-Term
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适用
- 人, 大鼠, Pig, 猴, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This S1PR5 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human S1PR5 / EDG8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Elephant, Bat, Pig (100%) Mouse, Rat, Hamster, Dog, Bovine (94%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from N-terminal extracellular domain of human S1PR5 / EDG8. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Elephant, Bat, Pig (100%), Mouse, Rat, Hamster, Dog, Bovine (94%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product S1PR5 Primary Antibody
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anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) (AA 1-200) antibody
S1PR5 适用: 小鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) (AA 1-50) antibodyS1PR5 适用: 人, 小鼠, 大鼠, 犬, Cow, 猴, Opossum WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) (AA 309-398) antibodyS1PR5 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) (C-Term) antibodyS1PR5 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) (N-Term) antibodyS1PR5 适用: 人, 小鼠, 大鼠, 犬, Pig, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) (N-Term) antibodyS1PR5 适用: 人, 小鼠, 大鼠, 犬, Pig, 猴, Bat, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) (N-Term) antibodyS1PR5 适用: 人, 小鼠, 大鼠, 犬, Drosophila melanogaster, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) (N-Term) antibodyS1PR5 适用: 人, 小鼠, 大鼠, 犬, Pig, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) (AA 24-39), (Extracellular), (N-Term) antibodyS1PR5 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 5 (S1PR5) antibodyS1PR5 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (3 - 6 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))
- 别名
- S1PR5 / EDG8 / S1P5 (S1PR5 产品)
- 别名
- EDG8 antibody, Edg-8 antibody, S1P5 antibody, SPPR-1 antibody, SPPR-2 antibody, fd11a07 antibody, wu:fd11a07 antibody, zgc:92296 antibody, edg-8 antibody, edg8 antibody, s1p5 antibody, sppr-1 antibody, sppr-2 antibody, xts1p5 antibody, Edg8 antibody, lpB4 antibody, zgc:158362 antibody, sphingosine-1-phosphate receptor 5 antibody, sphingosine-1-phosphate receptor 5a antibody, sphingosine-1-phosphate receptor 5b antibody, S1PR5 antibody, s1pr5a antibody, s1pr5 antibody, S1pr5 antibody, s1pr5b antibody
- 背景
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Name/Gene ID: S1PR5
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR
Synonyms: S1PR5, EDG8, Edg-8, S1P receptor Edg-8, S1P receptor 5, S1P(5) receptor, S1P5, SPPR-1, SPPR-2 - 基因ID
- 53637
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