RXFP2 抗体 (Cytoplasmic Domain)
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- 抗原 See all RXFP2 抗体
- RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 小鼠, 马
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RXFP2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Human RXFP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 交叉反应
- 马
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%) Monkey, Panda (93%) Dog, Pig, Xenopus (86%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 14 amino acid peptide from 2nd cytoplasmic domain of human RXFP2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%), Monkey, Panda (93%), Dog, Pig, Xenopus (86%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product RXFP2 Primary Antibody
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anti-Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2) (AA 33-158) antibody
RXFP2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2) (Extracellular Domain) antibodyRXFP2 适用: 人, 小鼠 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2) (N-Term) antibodyRXFP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2) (C-Term) antibodyRXFP2 适用: 人, 猴 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2) antibodyRXFP2 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2) (Internal Region) antibodyRXFP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2) (AA 80-180) antibodyRXFP2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 - 10 μg/mL), WB
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))
- 别名
- RXFP2 / LGR8 (RXFP2 产品)
- 别名
- GPR106 antibody, GREAT antibody, INSL3R antibody, LGR8 antibody, LGR8.1 antibody, RXFPR2 antibody, Gpcr antibody, Lgr8 antibody, Gpr106 antibody, Great antibody, lgr8.1 antibody, rxfp2 antibody, rxfp2b antibody, zgc:63668 antibody, relaxin/insulin like family peptide receptor 2 antibody, relaxin/insulin-like family peptide receptor 2 antibody, relaxin/insulin-like family peptide receptor 2a antibody, RXFP2 antibody, Rxfp2 antibody, rxfp2a antibody
- 背景
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Name/Gene ID: RXFP2
Subfamily: Relaxin
Family: GPCR
Synonyms: RXFP2, G-protein coupled receptor 106, GPR106, INSL3R, LGR8, GREAT, Relaxin receptor 2, LGR8.1, RXFPR2 - 基因ID
- 122042
- 途径
- cAMP Metabolic Process, Myometrial Relaxation and Contraction
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