RORC 抗体 (Ligand Binding Domain)
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- 抗原 See all RORC 抗体
- RORC (RAR-Related Orphan Receptor C (RORC))
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抗原表位
- Ligand Binding Domain
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RORC antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ROR Gamma. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 无交叉反应
- 猕猴
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Mouse, Hamster, Rabbit (94%) Rat, Elephant, Panda, Bat, Bovine, Horse (89%) Dog (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from ligand-binding domain of human ROR Gamma. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Hamster, Rabbit (94%), Rat, Elephant, Panda, Bat, Bovine, Horse (89%), Dog (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product RORC Primary Antibody
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anti-RAR-Related Orphan Receptor C (RORC) (AA 212-461) antibody
RORC 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor C (RORC) (AA 200-212) antibodyVerified RORC 适用: 人 WB, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor C (RORC) (N-Term) antibodyRORC 适用: 人, 小鼠, 大鼠, Cow, 马, 犬, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor C (RORC) (Middle Region) antibodyRORC 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor C (RORC) (AA 412-517) antibodyRORC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-RAR-Related Orphan Receptor C (RORC) (AA 110-159) antibodyRORC 适用: 人, Cow, 马, 豚鼠, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor C (RORC) (AA 1-50) antibodyRORC 适用: 人, 小鼠 WB, FACS 宿主: 小鼠 Monoclonal 4G419 unconjugated
anti-RAR-Related Orphan Receptor C (RORC) (AA 1-518) antibodyRORC 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor C (RORC) antibodyRORC 适用: 人 ELISA, Coat 宿主: 小鼠 Monoclonal RORC-2942 unconjugated
anti-RAR-Related Orphan Receptor C (RORC) (Ligand Binding Domain) antibodyRORC 适用: 人, 猴 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (20 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- RORC (RAR-Related Orphan Receptor C (RORC))
- 别名
- RORC / ROR gamma (RORC 产品)
- 别名
- NR1F3 antibody, RORG antibody, RZR-GAMMA antibody, RZRG antibody, TOR antibody, Nr1f3 antibody, RORgamma antibody, Thor antibody, RAR related orphan receptor C antibody, RAR-related orphan receptor C antibody, RAR-related orphan receptor gamma antibody, RORC antibody, Rorc antibody
- 背景
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Name/Gene ID: RORC
Subfamily: NR1 Thyroid hormone-like
Family: NHR
Synonyms: RORC, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, RAR-related orphan receptor C, RORG, RORgamma, TOR, ROR Gamma, RZRG, NR1F3, RZR-GAMMA - 基因ID
- 6097
- UniProt
- P51449
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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