RORA 抗体 (Ligand Binding Domain)
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- 抗原 See all RORA 抗体
- RORA (RAR-Related Orphan Receptor A (RORA))
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抗原表位
- Ligand Binding Domain
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适用
- 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 猴, Pig, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RORA antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ROR Alpha. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%) Turkey, Chicken, Xenopus (95%) Stickleback, Pufferfish, Zebrafish (90%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from ligand-binding domain of human ROR Alpha. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%), Turkey, Chicken, Xenopus (95%), Stickleback, Pufferfish, Zebrafish (90%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product RORA Primary Antibody
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anti-RAR-Related Orphan Receptor A (RORA) antibody
KO Validated RORA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor A (RORA) (Middle Region) antibodyRORA 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, 山羊, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor A (RORA) (Hinge Region) antibodyRORA 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 猴, Pig, Bat, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor A (RORA) (N-Term) antibodyRORA 适用: 人, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor A (RORA) (AA 193-222) antibodyRORA 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB16632 unconjugated
anti-RAR-Related Orphan Receptor A (RORA) antibodyRORA 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor A (RORA) (AA 1-468) antibodyRORA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor A (RORA) (AA 50-99) antibodyRORA 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor A (RORA) (AA 239-288) antibodyRORA 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, 猴, 山羊, Pig, 斑马鱼, Bat, 绵羊, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAR-Related Orphan Receptor A (RORA) (AA 36-85) antibodyRORA 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (3 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- RORA (RAR-Related Orphan Receptor A (RORA))
- 别名
- RORA / ROR alpha (RORA 产品)
- 别名
- ror1 antibody, ror2 antibody, ror3 antibody, rzra antibody, nr1f1 antibody, MGC146531 antibody, NR1F1 antibody, ROR1 antibody, ROR2 antibody, ROR3 antibody, RZR-ALPHA antibody, RZRA antibody, RORalpha-B antibody, gb:dq017624 antibody, rora2 antibody, 9530021D13Rik antibody, Nr1f1 antibody, nmf267 antibody, sg antibody, staggerer antibody, tmgc26 antibody, RORalpha1 antibody, RAR related orphan receptor A antibody, RAR-related orphan receptor A antibody, RAR-related orphan receptor A, paralog a antibody, RAR-related orphan receptor alpha antibody, RORA antibody, rora antibody, Rora antibody, roraa antibody
- 背景
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Name/Gene ID: RORA
Subfamily: NR1 Thyroid hormone-like
Family: NHR
Synonyms: RORA, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, Roralpha, Transcription factor RZR-alpha, RAR-related orphan receptor A, ROR-alpha, ROR3, RZRA, NR1F1, ROR Alpha, RZR-ALPHA - 基因ID
- 6095
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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