-
- 抗原 See all PYGB (GPBB) 抗体
- PYGB (GPBB) (phosphorylase, Glycogen, Brain (GPBB))
-
抗原表位
- C-Term
-
适用
- 人, 兔, 猴
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This PYGB antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human PYGB. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rabbit (100%) Orangutan, Mouse, Rat, Dog, Hamster, Elephant, Panda, Horse (92%) Turkey, Chicken, Platypus, Xenopus (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 12 amino acid peptide from C-terminus of human PYGB. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rabbit (100%), Orangutan, Mouse, Rat, Dog, Hamster, Elephant, Panda, Horse (92%), Turkey, Chicken, Platypus, Xenopus (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GPBB Primary Antibody
-
anti-phosphorylase, Glycogen, Brain (GPBB) (C-Term) antibody
GPBB 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Brain (GPBB) (AA 477-731) antibodyGPBB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Brain (GPBB) (AA 544-843) antibodyGPBB 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Brain (GPBB) (AA 6-843) antibodyGPBB 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Brain (GPBB) antibodyGPBB 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Brain (GPBB) (C-Term) antibodyGPBB 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Brain (GPBB) antibodyGPBB 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Brain (GPBB) antibodyGPBB 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Brain (GPBB) (AA 180-229) antibodyGPBB 适用: 人, 小鼠, 兔, 绵羊, Cow, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Brain (GPBB) (AA 1-843) antibodyGPBB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- PYGB (GPBB) (phosphorylase, Glycogen, Brain (GPBB))
- 别名
- GPBB / PYGB (GPBB 产品)
- 别名
- GPBB antibody, GLYPHOA antibody, glycogen phosphorylase B antibody, phosphorylase, glycogen; brain antibody, brain glycogen phosphorylase antibody, phosphorylase, glycogen; brain S homeolog antibody, PYGB antibody, pygb antibody, Pygb antibody, pygb.S antibody
- 背景
-
Name/Gene ID: PYGB
Synonyms: PYGB, GPBB, Phosphorylase, glycogen, Phosphorylase, glycogen brain, Glycogen phosphorylase B - 基因ID
- 5834
- 途径
- Cellular Glucan Metabolic Process
-
You are here:
