PAK6 抗体 (Linker Domain)
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- 抗原 See all PAK6 抗体
- PAK6 (P21-Activated Kinase 6 (PAK6))
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抗原表位
- Linker Domain
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适用
- 人, 马, 犬, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PAK6 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human PAK6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Horse (100%) Goat, Elephant, Panda, Rabbit (94%) Hamster, Bovine (89%) Mouse, Bat (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from Linker domain of human PAK6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Horse (100%), Goat, Elephant, Panda, Rabbit (94%), Hamster, Bovine (89%), Mouse, Bat (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product PAK6 Primary Antibody
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anti-P21-Activated Kinase 6 (PAK6) (AA 268-297) antibody
PAK6 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 6 (PAK6) (AA 1-681) antibodyPAK6 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 6 (PAK6) (AA 268-297) antibodyPAK6 适用: 人 WB, IHC (p), IF 宿主: 兔 Polyclonal RB31991 unconjugated
anti-P21-Activated Kinase 6 (PAK6) (AA 1-270) antibodyPAK6 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 6 (PAK6) (N-Term) antibodyPAK6 适用: 人, 小鼠, 大鼠, 马, Cow, 犬, 兔, 猴, Bat, 小鸡 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 6 (PAK6) (AA 1-270) antibodyPAK6 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 6 (PAK6) (N-Term) antibodyPAK6 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 6 (PAK6) (AA 275-305), (Middle Region) antibodyPAK6 适用: 人 WB, IHC (p), IF, EIA 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 6 (PAK6) antibodyPAK6 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 6 (PAK6) (AA 16-32), (N-Term) antibodyPAK6 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (3.5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- PAK6 (P21-Activated Kinase 6 (PAK6))
- 别名
- PAK6 (PAK6 产品)
- 别名
- PAK5 antibody, 4732456M09 antibody, pak6 antibody, si:ch211-158b22.3 antibody, p21 (RAC1) activated kinase 6 antibody, p21 protein (Cdc42/Rac)-activated kinase 6 antibody, p21 protein (Cdc42/Rac)-activated kinase 6b antibody, PAK6 antibody, Pak6 antibody, pak6b antibody
- 背景
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Name/Gene ID: PAK6
Subfamily: PAK (STE20)
Family: Protein Kinase
Synonyms: PAK6, PAK5, p21-activated kinase 6, p21-activated protein kinase 6, PAK-5, PAK-6, p21(CDKN1A)-activated kinase 6 - 基因ID
- 56924
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