P2RY6 抗体 (C-Term)
-
- 抗原 See all P2RY6 抗体
- P2RY6 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6))
-
抗原表位
- C-Term
-
适用
- 人, 猴
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This P2RY6 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human P2RY6 / P2Y6. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HTT (59 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda (100%) Dog, Pig (94%) Hamster, Bovine, Bat (88%) Rat, Rabbit (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 17 amino acid peptide from C-terminus of human P2RY6 / P2Y6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda (100%), Dog, Pig (94%), Hamster, Bovine, Bat (88%), Rat, Rabbit (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product P2RY6 Primary Antibody
-
anti-Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6) (C-Term) antibody
P2RY6 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6) (2nd Extracellular Loop), (AA 172-186) antibodyP2RY6 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6) antibodyP2RY6 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6) (Cytoplasmic Domain) antibodyP2RY6 适用: 人, 大鼠, Cow, Pig, 兔, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6) (AA 109-158) antibodyP2RY6 适用: 人, 大鼠, Cow, Pig, 兔, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6) (AA 311-328), (C-Term), (Intracellular) antibodyP2RY6 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6) (AA 172-186), (Extracellular), (Loop 2) antibody (PE)P2RY6 适用: 人, 大鼠, 小鼠 FACS 宿主: 兔 Polyclonal PE
anti-Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6) (2nd Extracellular Loop), (AA 172-186) antibody (FITC)P2RY6 适用: 人, 大鼠, 小鼠 FACS 宿主: 兔 Polyclonal FITC
-
- 应用备注
- Approved: ICC (8 - 16 μg/mL), IHC, IHC-P (3 - 16 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- P2RY6 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6))
- 别名
- P2RY6 / P2Y6 (P2RY6 产品)
- 别名
- P2Y6 antibody, 2010204J23Rik antibody, P2y6 antibody, P2RY3 antibody, P2Y3 antibody, P2Y6R antibody, pyrimidinergic receptor P2Y6 antibody, pyrimidinergic receptor P2Y, G-protein coupled, 6 antibody, P2RY6 antibody, P2ry6 antibody
- 背景
-
Name/Gene ID: P2RY6
Subfamily: Purinergic
Family: GPCR
Synonyms: P2RY6, G-coupled nucleotide receptor, p2 purinoceptor, p2y3 receptor, p2Y purinoceptor 6, Purinergic receptor p2y6, p2RY6, p2y3 and chick, p2Y6 receptor, p2Y6, Purinoceptor p2y6 - 基因ID
- 5031
- 途径
- Smooth Muscle Cell Migration
-
