P2RY1 抗体 (C-Term)
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- 抗原 See all P2RY1 抗体
- P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))
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抗原表位
- C-Term
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适用
- 人, 大鼠, 小鼠, Cow, 豚鼠, 兔, Bat, 犬, 猴, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This P2RY1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human P2RY1 / P2Y1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Bovine, Rabbit, Opossum, Guinea pig (100%) Horse, Xenopus (93%) Lizard (87%) Turkey, Chicken (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from C-terminus of human P2RY1 / P2Y1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Bovine, Rabbit, Opossum, Guinea pig (100%), Horse, Xenopus (93%), Lizard (87%), Turkey, Chicken (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product P2RY1 Primary Antibody
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anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) antibody
P2RY1 适用: 人 ELISA, WB, IHC, FACS 宿主: 小鼠 Monoclonal 4G5D6 unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) (AA 1-52) antibodyP2RY1 适用: 人 ELISA, WB, IF 宿主: 小鼠 Monoclonal 4C2 unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) antibodyP2RY1 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) (AA 71-120) antibodyP2RY1 适用: 人, 大鼠, 小鼠, Cow, 豚鼠, 兔, Bat, 马, 犬, 猴, Hamster, 小鸡, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) (2nd Extracellular Loop), (AA 207-220) antibodyP2RY1 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, IF, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) (AA 329-373) antibodyP2RY1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) (C-Term) antibodyP2RY1 适用: 人, 大鼠, 小鼠, Cow, 豚鼠, 兔, Bat, 马, 犬, 猴, Hamster ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) (Extracellular Domain) antibodyP2RY1 适用: 人, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) (AA 1-373) antibodyP2RY1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1) (N-Term) antibodyP2RY1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: ICC, IHC, IHC-P (2 - 5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))
- 别名
- P2RY1 / P2Y1 (P2RY1 产品)
- 别名
- P2Y1 antibody, P2y antibody, P2y1 antibody, purinergic receptor P2Y, G-protein coupled 1 antibody, purinergic receptor P2Y1 antibody, P2ry1 antibody, P2RY1 antibody
- 背景
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Name/Gene ID: P2RY1
Subfamily: Purinergic
Family: GPCR
Synonyms: P2RY1, ATP receptor, p2Y1, p2y1r, Platelet ADP receptor, Purinoceptor p2y1, Purinergic receptor, p2 purinoceptor subtype Y1, p2RY1, p2Y purinoceptor 1, p2y-1-r, Purinoceptor p2yr1, p2y1 receptor - 基因ID
- 5028
- 途径
- Regulation of Carbohydrate Metabolic Process, Feeding Behaviour
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