NPY5R 抗体 (Cytoplasmic Domain)
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- 抗原 See all NPY5R 抗体
- NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NPY5R antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 品牌
- IHC-plus™
- 特异性
- Human NPY5R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Bovine, Panda (88%) Dog, Rabbit, Pig (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from 3rd cytoplasmic domain of human NPY5R. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Bovine, Panda (88%), Dog, Rabbit, Pig (82%).
Type of Immunogen: Synthetic peptide
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anti-Neuropeptide Y Receptor Y5 (NPY5R) (Cytoplasmic Domain) antibody
NPY5R 适用: 人 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y5 (NPY5R) (Internal Region) antibodyNPY5R 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y5 (NPY5R) (Internal Region) antibodyVerified NPY5R 适用: 人, Hamster WB, IHC, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y5 (NPY5R) (AA 1-445) antibodyNPY5R 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y5 (NPY5R) (Cytoplasmic Domain) antibodyNPY5R 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y5 (NPY5R) (AA 221-270) antibodyNPY5R 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y5 (NPY5R) (3rd Intracellular Loop), (AA 291-306) antibodyNPY5R 适用: 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y5 (NPY5R) (AA 1-100) antibodyNPY5R 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y5 (NPY5R) (Internal Region) antibodyNPY5R 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y5 (NPY5R) (AA 305-341) antibodyNPY5R 适用: 人 WB 宿主: 兔 Polyclonal RB52205 unconjugated
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- 应用备注
- Approved: ICC (4 - 6 μg/mL), IHC, IHC-P (4 - 8 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))
- 别名
- NPY5R (NPY5R 产品)
- 别名
- NPY5-R antibody, NPYR5 antibody, NPYY5-R antibody, Y5R antibody, NPY5 antibody, MGC146705 antibody, NPY5R antibody, neuropeptide Y receptor Y5 antibody, neuropeptide Y receptor type 5 antibody, NPY5R antibody, Npy5r antibody, npy5r antibody, LOC786150 antibody
- 背景
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Name/Gene ID: NPY5R
Subfamily: Neuropeptide
Family: GPCR
Synonyms: NPY5R, NPYR5, NPY5-R, NPYY5, Neuropeptide Y receptor Y5, NPYY5-R, Neuropeptide Y receptor type 5, Neuropeptide Y5 receptor, NPY-Y5 receptor, NPY5 receptor, Y5 receptor - 基因ID
- 4889
- 途径
- Feeding Behaviour
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