电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

NPY1R 抗体 (Cytoplasmic Domain)

NPY1R 适用: 人, 大鼠, 小鼠, 马, 猴, 犬, 豚鼠, 兔, Bat, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1049122
发货至: 中国
  • 抗原 See all NPY1R 抗体
    NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))
    抗原表位
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    适用
    人, 大鼠, 小鼠, 马, 猴, 犬, 豚鼠, 兔, Bat, Pig
    宿主
    • 52
    • 7
    • 1
    • 1
    克隆类型
    • 56
    • 5
    多克隆
    标记
    • 36
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This NPY1R antibody is un-conjugated
    应用范围
    • 38
    • 25
    • 12
    • 8
    • 8
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human NPY1R. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except NPY6R (61 % ).
    预测反应
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Dog, Panda, Rabbit, Horse, Pig, Guinea pig (100%) Sheep, Hamster, Elephant (94%) Bovine, Lizard (89%) Xenopus (83%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human NPY1R. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Dog, Panda, Rabbit, Horse, Pig, Guinea pig (100%), Sheep, Hamster, Elephant (94%), Bovine, Lizard (89%), Xenopus (83%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product NPY1R Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (16 μg/mL)
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))
    别名
    NPY1R (NPY1R 产品)
    别名
    NPY1-R antibody, NPYR antibody, NPY-1 antibody, NPY1R antibody, si:dkey-253i9.3 antibody, Npyr antibody, Y1-R antibody, npy1r-A antibody, npyr antibody, neuropeptide Y receptor Y1 antibody, neuropeptide Y receptor Y1 S homeolog antibody, NPY1R antibody, Npy1r antibody, npy1r antibody, npy1r.S antibody
    背景
    Name/Gene ID: NPY1R
    Subfamily: Neuropeptide
    Family: GPCR

    Synonyms: NPY1R, NPYY1, NPY-Y1 receptor, Neuropeptide Y receptor type 1, PY receptor Y1, Neuropeptide Y receptor Y1, Neuropeptide Y-Y1 receptor, Neuropeptide Y1 receptor, NPY1-R, NPYR
    基因ID
    4886
    途径
    Negative Regulation of Hormone Secretion, Feeding Behaviour
You are here:
客服