NPY1R 抗体 (Extracellular Domain)
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- 抗原 See all NPY1R 抗体
- NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))
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抗原表位
- Extracellular Domain
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NPY1R antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NPY1R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from 2nd extracellular domain of human NPY1R. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Sheep, Bovine, Dog, Elephant, Panda, Pig (94%), Hamster, Rabbit, Guinea pig (89%), Mouse, Rat, Horse (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NPY1R Primary Antibody
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anti-Neuropeptide Y Receptor Y1 (NPY1R) (AA 301-382) antibody
NPY1R 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y1 (NPY1R) (Middle Region) antibodyNPY1R 适用: 人, 大鼠, 小鼠, 马, 犬, 豚鼠, 兔, Cow, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y1 (NPY1R) antibodyNPY1R 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y1 (NPY1R) (AA 1-115) antibodyNPY1R 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Neuropeptide Y Receptor Y1 (NPY1R) (AA 180-229) antibodyNPY1R 适用: 人, 大鼠, 小鼠, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y1 (NPY1R) (Internal Region) antibodyNPY1R 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y1 (NPY1R) (2nd Extracellular Loop), (AA 197-210) antibodyNPY1R 适用: 人, 大鼠, 小鼠 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y1 (NPY1R) (Cytoplasmic Domain) antibodyNPY1R 适用: 人, 大鼠, 小鼠, 马, 猴, 犬, 豚鼠, 兔, Bat, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y1 (NPY1R) (C-Term) antibodyNPY1R 适用: 人, 大鼠, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide Y Receptor Y1 (NPY1R) (3rd Intracellular Loop), (AA 237-252) antibodyNPY1R 适用: 人, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 - 10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))
- 别名
- NPY1R (NPY1R 产品)
- 别名
- NPY1-R antibody, NPYR antibody, NPY-1 antibody, NPY1R antibody, si:dkey-253i9.3 antibody, Npyr antibody, Y1-R antibody, npy1r-A antibody, npyr antibody, neuropeptide Y receptor Y1 antibody, neuropeptide Y receptor Y1 S homeolog antibody, NPY1R antibody, Npy1r antibody, npy1r antibody, npy1r.S antibody
- 背景
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Name/Gene ID: NPY1R
Subfamily: Neuropeptide
Family: GPCR
Synonyms: NPY1R, NPYY1, NPY-Y1 receptor, Neuropeptide Y receptor type 1, PY receptor Y1, Neuropeptide Y receptor Y1, Neuropeptide Y-Y1 receptor, Neuropeptide Y1 receptor, NPY1-R, NPYR - 基因ID
- 4886
- 途径
- Negative Regulation of Hormone Secretion, Feeding Behaviour
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