NPFFR1 抗体 (Extracellular Domain)
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- 抗原 See all NPFFR1 抗体
- NPFFR1 (Neuropeptide FF Receptor 1 (NPFFR1))
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抗原表位
- Extracellular Domain
- 适用
- 人, Cow, 马, 猴, Pig, 兔
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NPFFR1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NPFF1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Horse, Rabbit, Pig (100%) Mouse, Rat, Dog, Panda (95%) Elephant, Turkey, Chicken (85%) Opossum, Platypus (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from 2nd extracellular domain of human NPFF1 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Horse, Rabbit, Pig (100%), Mouse, Rat, Dog, Panda (95%), Elephant, Turkey, Chicken (85%), Opossum, Platypus (80%).
Type of Immunogen: Synthetic peptide
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anti-Neuropeptide FF Receptor 1 (NPFFR1) (Internal Region) antibody
Verified NPFFR1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (19 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NPFFR1 (Neuropeptide FF Receptor 1 (NPFFR1))
- 别名
- NPFFR1 / GPR147 (NPFFR1 产品)
- 别名
- GPR147 antibody, NPFF1 antibody, NPFF1R1 antibody, OT7T022 antibody, Gm236 antibody, Gpr147 antibody, RFRPR antibody, MGC83178 antibody, NPFFR1 antibody, LOC100230250 antibody, neuropeptide FF receptor 1 antibody, neuropeptide FF receptor 1 L homeolog antibody, inorganic pyrophosphatase-like antibody, NPFFR1 antibody, Npffr1 antibody, npffr1.L antibody, LOC100230250 antibody
- 背景
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Name/Gene ID: NPFFR1
Subfamily: Neuropeptide
Family: GPCR
Synonyms: NPFFR1, G-protein coupled receptor 147, GPR147, Neuropeptide FF 1, Neuropeptide FF receptor 1, NPFF-R1, OT7T022, NPFF1 receptor, G protein-coupled receptor 147, Neuropeptide FF1 receptor, NPFF1, NPFF1R1 - 基因ID
- 64106
- 途径
- cAMP Metabolic Process
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