TACR1 抗体 (C-Term)
-
- 抗原 See all TACR1 抗体
- TACR1 (Tachykinin Receptor 1 (TACR1))
-
抗原表位
- C-Term
-
适用
- 人, 大鼠, 豚鼠, 猴, 马, 兔, Hamster
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This TACR1 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human Neurokinin 1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DTNB (44 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rat, Hamster, Panda, Horse, Rabbit, Guinea pig (100%) Mouse, Dog, Elephant (94%) Marmoset, Bat, Bovine (89%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 18 amino acid peptide from C-terminus of human Neurokinin 1 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rat, Hamster, Panda, Horse, Rabbit, Guinea pig (100%), Mouse, Dog, Elephant (94%), Marmoset, Bat, Bovine (89%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product TACR1 Primary Antibody
-
anti-Tachykinin Receptor 1 (TACR1) (AA 2-50) antibody
TACR1 适用: 人, 大鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Tachykinin Receptor 1 (TACR1) (Internal Region) antibodyVerified TACR1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Tachykinin Receptor 1 (TACR1) (2nd Extracellular Loop), (AA 180-194) antibodyTACR1 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-Tachykinin Receptor 1 (TACR1) (2nd Extracellular Loop), (AA 180-194) antibody (Atto 488)TACR1 适用: 人, 大鼠, 小鼠 IHC, IF, FACS 宿主: 兔 Polyclonal Atto 488
anti-Tachykinin Receptor 1 (TACR1) (AA 1-29) antibodyTACR1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tachykinin Receptor 1 (TACR1) (AA 140-243) antibodyTACR1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Tachykinin Receptor 1 (TACR1) (Extracellular Domain) antibodyTACR1 适用: 人, 犬, 猴, Cow IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tachykinin Receptor 1 (TACR1) antibodyMicroarray verified TACR1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB323175 unconjugated
anti-Tachykinin Receptor 1 (TACR1) antibodyMicroarray verified TACR1 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB139563 unconjugated
anti-Tachykinin Receptor 1 (TACR1) (Internal Region) antibodyTACR1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (1 - 40 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- TACR1 (Tachykinin Receptor 1 (TACR1))
- 别名
- TACR1 / NK1R (TACR1 产品)
- 别名
- NK1R antibody, NKIR antibody, SPR antibody, TAC1R antibody, Nk1r antibody, Spr antibody, Tac1r antibody, nk1R antibody, Tacr1 antibody, ASPR antibody, tachykinin receptor 1 antibody, TACR1 antibody, Tacr1 antibody
- 背景
-
Name/Gene ID: TACR1
Subfamily: Tachykinin
Family: GPCR
Synonyms: TACR1, Neurokinin receptor 1, NK-1R, Nk1 tachykinin receptor, NK1R, TAC1R, Tachykinin 1 receptor, NKIR, Substance-P receptor, Neurokinin 1 receptor, NK-1 receptor, Tachykinin receptor 1 - 基因ID
- 6869
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
-
