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- 抗原 See all NEK6 抗体
- NEK6 (NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6))
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抗原表位
- N-Term
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NEK6 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NEK6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (86%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 14 amino acid peptide from N-terminus of human NEK6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (86%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NEK6 Primary Antibody
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anti-NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) antibody
NEK6 适用: 人 WB, IHC, IHC (p), IP 宿主: 小鼠 Monoclonal 2H7 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) antibodyNEK6 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (AA 1-100) antibodyNEK6 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (AA 251-280), (C-Term) antibodyNEK6 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB34648 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (AA 1-100) antibodyNEK6 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 3B5 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (AA 38-87) antibodyNEK6 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 猴, Bat, 小鸡, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (AA 8-39), (N-Term) antibodyNEK6 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB2965 unconjugated
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (N-Term) antibodyNEK6 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 7 μg/mL. - 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NEK6 (NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6))
- 别名
- NEK6 (NEK6 产品)
- 别名
- 1300007C09Rik antibody, si:ch211-167p9.4 antibody, ATNEK5 antibody, MOE17.17 antibody, NIMA-RELATED KINASE5 antibody, NIMA-related kinase 5 antibody, SID6-1512 antibody, NIMA (never in mitosis gene a)-related expressed kinase 6 antibody, NIMA-related kinase 6 antibody, NIMA related kinase 6 antibody, Serine/Threonine kinase catalytic domain protein antibody, NIMA-related kinase 6 S homeolog antibody, Nek6 antibody, nek6 antibody, NEK6 antibody, NEK5 antibody, nek6.S antibody
- 背景
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Name/Gene ID: NEK6
Subfamily: NEK
Family: Protein Kinase
Synonyms: NEK6, NIMA-related kinase 6, Protein kinase SID6-1512, NimA-related protein kinase 6, SID6-1512 - 基因ID
- 10783
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