NANP 抗体 (N-Term)
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- 抗原 See all NANP 抗体
- NANP (N-Acetylneuraminic Acid Phosphatase (NANP))
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抗原表位
- N-Term
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适用
- 人, 大鼠, 小鼠, 猴, Cow, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NANP antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NANP. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat (100%) Hamster, Pig (94%) Elephant, Panda, Dog, Rabbit (88%) Turkey, Chicken (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from near N-terminus of human NANP. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat (100%), Hamster, Pig (94%), Elephant, Panda, Dog, Rabbit (88%), Turkey, Chicken (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NANP Primary Antibody
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anti-N-Acetylneuraminic Acid Phosphatase (NANP) antibody
NANP 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Acetylneuraminic Acid Phosphatase (NANP) (AA 1-248) antibodyNANP 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-N-Acetylneuraminic Acid Phosphatase (NANP) (Internal Region) antibodyNANP 适用: 人, 大鼠, 小鼠, 兔, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-N-Acetylneuraminic Acid Phosphatase (NANP) (Internal Region) antibodyNANP 适用: 人, 大鼠, 小鼠, 兔, 犬, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-N-Acetylneuraminic Acid Phosphatase (NANP) antibodyNANP 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3C1 unconjugated
anti-N-Acetylneuraminic Acid Phosphatase (NANP) antibodyNANP 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4F5 unconjugated
anti-N-Acetylneuraminic Acid Phosphatase (NANP) (AA 1-248) antibodyNANP 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Acetylneuraminic Acid Phosphatase (NANP) (AA 179-228) antibodyNANP 适用: 人, 大鼠, 小鼠, 兔, 猴, 犬, Cow, Hamster, 豚鼠, Pig, 斑马鱼, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-N-Acetylneuraminic Acid Phosphatase (NANP) (AA 1-248) antibodyNANP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-N-Acetylneuraminic Acid Phosphatase (NANP) (Middle Region) antibodyNANP 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (6 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NANP (N-Acetylneuraminic Acid Phosphatase (NANP))
- 别名
- NANP (NANP 产品)
- 别名
- rgd1306009 antibody, C20orf147 antibody, HDHD4 antibody, dJ694B14.3 antibody, 1600031M04Rik antibody, Hdhd4 antibody, RGD1306009 antibody, zgc:111947 antibody, N-acylneuraminate-9-phosphatase antibody, N-acetylneuraminic acid phosphatase antibody, N-acetylneuraminic acid phosphatase L homeolog antibody, nanp antibody, NANP antibody, Nanp antibody, nanp.L antibody
- 背景
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Name/Gene ID: NANP
Family: Phosphatase
Synonyms: NANP, C20orf147, DJ694B14.3, Neu5Ac-9-Pase, HDHD4 - 基因ID
- 140838
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