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- 抗原 See all MST1R 抗体
- MST1R (Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R))
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抗原表位
- N-Term
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MST1R antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human MST1R / RON. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Gibbon, Dog (94%) Rabbit (89%) Panda, Bovine (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from N-terminus of human MST1R / RON. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Gibbon, Dog (94%), Rabbit (89%), Panda, Bovine (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MST1R Primary Antibody
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anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (AA 89-275) antibody
MST1R 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (pTyr1238) antibodyMST1R 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (AA 1092-1316) antibodyMST1R 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (C-Term) antibodyMST1R 适用: 人, 小鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (AA 210-320) antibodyMST1R 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B5A9 unconjugated
anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (pTyr1238), (pTyr1239) antibodyMST1R 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (pSer1394) antibodyMST1R 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (N-Term) antibodyMST1R 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (AA 1175-1400) antibodyMST1R 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) (AA 600-633) antibodyMST1R 适用: 人 WB 宿主: 兔 Polyclonal RB58004 unconjugated
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- 应用备注
- Approved: IHC, IHC-P (2.5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- MST1R (Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R))
- 别名
- MST1R / RON (MST1R 产品)
- 别名
- CD136 antibody, CDw136 antibody, PTK8 antibody, RON antibody, Fv-2 antibody, Fv2 antibody, Ron antibody, STK antibody, Cdw136 antibody, Ptk8 antibody, Stk antibody, macrophage stimulating 1 receptor antibody, macrophage stimulating 1 receptor (c-met-related tyrosine kinase) antibody, MST1R antibody, Mst1r antibody
- 背景
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Name/Gene ID: MST1R
Subfamily: HGF Receptor/MET
Family: Protein Kinase
Synonyms: MST1R, C-met-related tyrosine kinase, CD136, CDw136, CD136 antigen, Friend virus susceptibility 2, Fv2, MSP receptor, p185-Ron, PTK8 protein tyrosine kinase 8, RON, Soluble RON variant 3, Soluble RON variant 1, MST1R variant RON30, Oncogene RON, PTK8, MST1R variant RON62, Protein-tyrosine kinase 8, RON variant E2E3, Soluble RON variant 2, Soluble RON variant 4 - 基因ID
- 4486
- 途径
- RTK signaling
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