MC4R 抗体 (N-Term)
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- 抗原 See all MC4R 抗体
- MC4R (Melanocortin 4 Receptor (MC4R))
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抗原表位
- N-Term
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适用
- 人, 猴, 马
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MC4R antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 品牌
- IHC-plus™
- 特异性
- Human MC4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Tamarin, Horse (100%) Panda, Guinea pig (94%) Mouse, Rat, Elephant, Pig (88%) Bat, Dog, Hamster (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from N-terminal extracellular domain of human MC4 Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Tamarin, Horse (100%), Panda, Guinea pig (94%), Mouse, Rat, Elephant, Pig (88%), Bat, Dog, Hamster (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MC4R Primary Antibody
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anti-Melanocortin 4 Receptor (MC4R) (AA 1-50) antibody
MC4R 适用: 人, 大鼠, 小鼠 ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 6-20), (Extracellular), (N-Term) antibodyMC4R 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 25-57) antibodyMC4R 适用: 斑马鱼 WB 宿主: 兔 Polyclonal RB52063 unconjugated
anti-Melanocortin 4 Receptor (MC4R) (Cytoplasmic Domain) antibodyMC4R 适用: 人, 绵羊, 兔, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (C-Term) antibodyMC4R 适用: 人, 大鼠, 小鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 21-33) antibodyMC4R 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (C-Term) antibodyMC4R 适用: 人, 大鼠, 小鼠, 绵羊, 兔, 猴, Cow, 犬, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 1-50) antibody (AbBy Fluor® 488)MC4R 适用: 人, 大鼠, 小鼠 IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 488
anti-Melanocortin 4 Receptor (MC4R) (AA 1-43) antibodyMC4R 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (N-Term) antibodyRecent MC4R 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- Approved: ICC (7.5 - 10 μg/mL), IHC, IHC-P (4 - 8 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- MC4R (Melanocortin 4 Receptor (MC4R))
- 别名
- MC4R / Melanocortin 4 Receptor (MC4R 产品)
- 别名
- zMC4 antibody, si:dkey-151m6.62 antibody, mc4r antibody, MC4R antibody, LOC100218148 antibody, Mc4-r antibody, melanocortin 4 receptor antibody, mc4r antibody, MC4R antibody, Mc4r antibody
- 背景
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Name/Gene ID: MC4R
Subfamily: Melanocortin
Family: GPCR
Synonyms: MC4R, Melanocortin receptor 4, MC4-R, MC4, MC4 receptor, Melanocortin 4 receptor - 基因ID
- 4160
- 途径
- Hormone Transport, cAMP Metabolic Process, Feeding Behaviour
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