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MC4R 抗体 (N-Term)

MC4R 适用: 人, 猴, 马 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1049046
发货至: 中国
  • 抗原 See all MC4R 抗体
    MC4R (Melanocortin 4 Receptor (MC4R))
    抗原表位
    • 15
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    • 5
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    • 1
    • 1
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    N-Term
    适用
    • 50
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    • 2
    • 2
    • 2
    • 2
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    人, 猴, 马
    宿主
    • 57
    • 1
    克隆类型
    • 58
    多克隆
    标记
    • 26
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    This MC4R antibody is un-conjugated
    应用范围
    • 27
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    • 6
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    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    品牌
    IHC-plus™
    特异性
    Human MC4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    预测反应
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Tamarin, Horse (100%) Panda, Guinea pig (94%) Mouse, Rat, Elephant, Pig (88%) Bat, Dog, Hamster (82%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic 17 amino acid peptide from N-terminal extracellular domain of human MC4 Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Tamarin, Horse (100%), Panda, Guinea pig (94%), Mouse, Rat, Elephant, Pig (88%), Bat, Dog, Hamster (82%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product MC4R Primary Antibody
  • 应用备注
    Approved: ICC (7.5 - 10 μg/mL), IHC, IHC-P (4 - 8 μg/mL)
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    MC4R (Melanocortin 4 Receptor (MC4R))
    别名
    MC4R / Melanocortin 4 Receptor (MC4R 产品)
    别名
    zMC4 antibody, si:dkey-151m6.62 antibody, mc4r antibody, MC4R antibody, LOC100218148 antibody, Mc4-r antibody, melanocortin 4 receptor antibody, mc4r antibody, MC4R antibody, Mc4r antibody
    背景
    Name/Gene ID: MC4R
    Subfamily: Melanocortin
    Family: GPCR

    Synonyms: MC4R, Melanocortin receptor 4, MC4-R, MC4, MC4 receptor, Melanocortin 4 receptor
    基因ID
    4160
    途径
    Hormone Transport, cAMP Metabolic Process, Feeding Behaviour
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