MARK1 抗体 (Internal Region)
-
- 抗原 See all MARK1 抗体
- MARK1 (MAP/microtubule Affinity-Regulating Kinase 1 (MARK1))
-
抗原表位
- Internal Region
-
适用
- 人, 猴, 兔, Bat
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This MARK1 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human MARK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Bat, Rabbit (100%) Rat, Panda, Dog, Bovine, Horse (94%) Mouse (88%) Platypus, Lizard (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 17 amino acid peptide from internal region of human MARK1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Bat, Rabbit (100%), Rat, Panda, Dog, Bovine, Horse (94%), Mouse (88%), Platypus, Lizard (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MARK1 Primary Antibody
-
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (AA 560-700) antibody
MARK1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (N-Term) antibodyMARK1 适用: 人, 猴, 兔, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (N-Term) antibodyMARK1 适用: 人, 猴, 兔, Bat, Cow, 犬 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (AA 6-40), (N-Term) antibodyMARK1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB8545 unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (Center) antibodyMARK1 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (AA 6-40) antibodyMARK1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) antibodyMARK1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) antibodyMARK1 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (AA 671-700), (C-Term) antibodyMARK1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB8547 unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (AA 643-674), (C-Term) antibodyMARK1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB1272 unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (2.5 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- MARK1 (MAP/microtubule Affinity-Regulating Kinase 1 (MARK1))
- 别名
- MARK1 / MARK (MARK1 产品)
- 别名
- MGC80341 antibody, MARK1 antibody, AW491150 antibody, B930025N23Rik antibody, Emk3 antibody, Par-1c antibody, mKIAA1477 antibody, mPar-1c antibody, MARK antibody, Par1c antibody, MAP/microtubule affinity-regulating kinase 1 S homeolog antibody, microtubule affinity regulating kinase 1 antibody, MAP/microtubule affinity-regulating kinase 1 antibody, MAP/microtubule affinity regulating kinase 1 antibody, mark1.S antibody, MARK1 antibody, mark1 antibody, Mark1 antibody
- 背景
-
Name/Gene ID: MARK1
Subfamily: MARK
Family: Protein Kinase
Synonyms: MARK1, KIAA1477, Par1c, PAR1 homolog c, MARK, Par-1c - 基因ID
- 4139
- 途径
- SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
-
