LINGO1 抗体 (C-Term)
-
- 抗原 See all LINGO1 抗体
- LINGO1 (Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1))
-
抗原表位
- C-Term
-
适用
- 人, 小鼠, 大鼠, Cow, 猴, Pig, 小鸡
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This LINGO1 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human LINGO1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Pig, Opossum, Turkey, Chicken (100%) Bat, Horse, Xenopus (94%) Dog (88%) Rabbit (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 16 amino acid peptide from C-terminus of human LINGO1. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Pig, Opossum, Turkey, Chicken (100%), Bat, Horse, Xenopus (94%), Dog (88%), Rabbit (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product LINGO1 Primary Antibody
-
anti-Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1) (AA 575-603), (C-Term) antibody
LINGO1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB15362 unconjugated
anti-Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1) (AA 521-620) antibodyLINGO1 适用: 小鼠, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1) (AA 42-240) antibodyLINGO1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1) (AA 62-92), (N-Term) antibodyLINGO1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB8949 unconjugated
anti-Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1) (AA 507-521), (Extracellular), (N-Term) antibodyLINGO1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1) (Internal Region) antibodyLINGO1 适用: 人, 小鼠, 大鼠, Cow, 猴, Pig, 犬, 马, 兔, 斑马鱼 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1) (AA 364-620) antibodyLINGO1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1) (N-Term) antibodyLINGO1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- LINGO1 (Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1))
- 别名
- LINGO1 (LINGO1 产品)
- 别名
- lern1 antibody, lrrn6a antibody, unq201 antibody, DKFZp459P0736 antibody, LERN1 antibody, LRRN6A antibody, UNQ201 antibody, 4930471K13Rik antibody, AV148400 antibody, LINGO-1 antibody, Lrrn6a antibody, leucine rich repeat and Ig domain containing 1 antibody, LINGO1 antibody, lingo1 antibody, Lingo1 antibody
- 背景
-
Name/Gene ID: LINGO1
Synonyms: LINGO1, LERN1, LRRN6A, UNQ201 - 基因ID
- 84894
-
