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- 抗原 See all LGR4 抗体
- LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))
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抗原表位
- Cytoplasmic Domain
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LGR4 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human LGR4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Panda (100%) Monkey, Rabbit, Pig (95%) Gibbon, Dog, Bovine (89%) Marmoset, Bat (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 19 amino acid peptide from 2nd cytoplasmic domain of human LGR4. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Panda (100%), Monkey, Rabbit, Pig (95%), Gibbon, Dog, Bovine (89%), Marmoset, Bat (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product LGR4 Primary Antibody
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anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) antibody
LGR4 适用: 人 FACS, cELISA 宿主: 大鼠 Monoclonal BBX-1H1 unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 234-365) antibodyLGR4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 852-950) antibodyLGR4 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 8F6 unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (C-Term) antibodyLGR4 适用: 人, 猴, 兔, Pig, Cow, 犬, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (C-Term) antibodyLGR4 适用: 人, 猴, 兔, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 320-540) antibodyLGR4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (Internal Region) antibodyLGR4 适用: 人, Chimpanzee, Macaque ELISA, WB, IHC 宿主: 小鼠 Monoclonal 6G8-B3-G5-C3 unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (N-Term) antibodyLGR4 适用: 人, 小鼠, 大鼠, 猴, 兔, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (C-Term) antibodyLGR4 适用: 人, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) antibodyLGR4 适用: 人, 小鼠 FACS, cELISA 宿主: 大鼠 Monoclonal BBX-3A8 unconjugated
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- 应用备注
- Approved: IHC, IHC-P (4 - 10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))
- 别名
- GPR48 / LGR4 (LGR4 产品)
- 别名
- BNMD17 antibody, GPR48 antibody, 9130225G07 antibody, A330106J01Rik antibody, A930009A08Rik antibody, Gpr48 antibody, leucine rich repeat containing G protein-coupled receptor 4 antibody, leucine-rich repeat-containing G protein-coupled receptor 4 antibody, LGR4 antibody, Lgr4 antibody
- 背景
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Name/Gene ID: LGR4
Subfamily: Orphan-A
Family: GPCR
Synonyms: LGR4, G-protein coupled receptor 48, G protein-coupled receptor 48, GPR48 - 基因ID
- 55366
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