ITGA3 抗体 (Internal Region)
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- 抗原 See all ITGA3 抗体
- ITGA3 (Integrin, alpha 3 (ITGA3))
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抗原表位
- Internal Region
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ITGA3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ITGA3 / CD49c. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%) Gibbon, Galago, Hamster, Elephant, Rabbit, Guinea pig (94%) Marmoset, Mouse, Rat, Panda, Bovine, Bat, Horse (89%) Pig (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from internal region of human ITGA3 / CD49c. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%), Gibbon, Galago, Hamster, Elephant, Rabbit, Guinea pig (94%), Marmoset, Mouse, Rat, Panda, Bovine, Bat, Horse (89%), Pig (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ITGA3 Primary Antibody
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anti-Integrin, alpha 3 (ITGA3) (AA 33-236) antibody
ITGA3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (AA 63-248) antibodyITGA3 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 7H2A11 unconjugated
anti-Integrin, alpha 3 (ITGA3) (AA 976-1025) antibodyITGA3 适用: 小鼠, Pig WB, ELISA, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) antibodyITGA3 适用: 人, 小鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (AA 168-276) antibodyITGA3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (AA 165-199), (N-Term) antibodyITGA3 适用: 人 WB 宿主: 兔 Polyclonal RB52792 unconjugated
anti-Integrin, alpha 3 (ITGA3) antibodyITGA3 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) antibodyITGA3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (Internal Region) antibodyITGA3 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (Cytoplasmic Domain) antibodyITGA3 适用: 人 WB, IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal 158A3 unconjugated
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- 应用备注
- Approved: IHC, IHC-P (2.5 - 5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- ITGA3 (Integrin, alpha 3 (ITGA3))
- 别名
- ITGA3 / CD49c (ITGA3 产品)
- 别名
- CD49C antibody, GAP-B3 antibody, GAPB3 antibody, ILNEB antibody, MSK18 antibody, VCA-2 antibody, VL3A antibody, VLA3a antibody, ITGA3 antibody, AA407068 antibody, integrin subunit alpha 3 antibody, integrin subunit alpha 3 S homeolog antibody, integrin alpha 3 antibody, ITGA3 antibody, itga3.S antibody, Itga3 antibody
- 背景
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Name/Gene ID: ITGA3
Family: Integrin
Synonyms: ITGA3, Alpha3 integrin, CD49c antigen, CD49C, GAPB3, Integrin alpha-3, FRP-2, VCA-2, VLA-3 subunit alpha, VL3A, VLA3a, Galactoprotein B3, GAP-B3, ILNEB, Integrin alpha3, MSK18 - 基因ID
- 3675
- 途径
- CXCR4-mediated Signaling Events, Integrin Complex
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