HRH4 抗体 (Cytoplasmic Domain)
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- 抗原 See all HRH4 抗体
- HRH4 (Histamine Receptor H4 (HRH4))
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HRH4 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 品牌
- IHC-plus™
- 特异性
- Human HRH4 / Histamine H4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset (100%) Bovine, Horse, Rabbit (94%) Gibbon, Mouse, Panda, Dog (89%) Rat, Opossum, Platypus (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from 1st cytoplasmic domain of human HRH4 / Histamine H4 Receptor. Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset (100%), Bovine, Horse, Rabbit (94%), Gibbon, Mouse, Panda, Dog (89%), Rat, Opossum, Platypus (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HRH4 Primary Antibody
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anti-Histamine Receptor H4 (HRH4) (C-Term) antibody
HRH4 适用: 人 ELISA, IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H4 (HRH4) (AA 204-292) antibodyHRH4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H4 (HRH4) (N-Term) antibodyKO Validated HRH4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H4 (HRH4) (AA 101-200) antibodyHRH4 适用: 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H4 (HRH4) (AA 285-390) antibodyHRH4 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H4 (HRH4) antibodyHRH4 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H4 (HRH4) (AA 359-390), (C-Term) antibodyHRH4 适用: 人 WB 宿主: 兔 Polyclonal RB1073 unconjugated
anti-Histamine Receptor H4 (HRH4) (AA 142-191) antibodyHRH4 适用: 人, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H4 (HRH4) (AA 194-304) antibodyHRH4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H4 (HRH4) (1st Extracellular Loop), (AA 75-87) antibodyHRH4 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: ICC, IF, IHC, IHC-P (12 - 24 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- HRH4 (Histamine Receptor H4 (HRH4))
- 别名
- HRH4 / Histamine H4 Receptor (HRH4 产品)
- 别名
- AXOR35 antibody, BG26 antibody, GPCR105 antibody, GPRv53 antibody, H4 antibody, H4R antibody, HH4R antibody, HRH4 antibody, histamine receptor H4 antibody, HRH4 antibody, Hrh4 antibody
- 背景
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Name/Gene ID: HRH4
Subfamily: Histamine
Family: GPCR
Synonyms: HRH4, BG26, G-protein coupled receptor 105, GPRv53, H4, HH4R, Histamine H4 receptor, GPCR105, H4R, Pfi-013, SP9144, AXOR35, Gprc105, H4 histamine receptor, Histamine receptor H4 - 基因ID
- 59340
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