GRM7 抗体 (N-Term)
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- 抗原 See all GRM7 抗体
- GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))
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抗原表位
- N-Term
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适用
- 人, Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GRM7 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 品牌
- IHC-plus™
- 特异性
- Human GRM7 / MGLUR7. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GRM8 (63 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Elephant, Bovine (100%) Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Dog, Horse (95%) Gibbon, Panda, Rabbit, Opossum (89%) Chicken, Xenopus (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 19 amino acid peptide from N-terminal extracellular domain of human GRM7 / MGLUR7. Percent identity with other species by BLAST analysis: Human, Elephant, Bovine (100%), Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Dog, Horse (95%), Gibbon, Panda, Rabbit, Opossum (89%), Chicken, Xenopus (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GRM7 Primary Antibody
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anti-Glutamate Receptor, Metabotropic 7 (GRM7) (AA 454-590) antibody
GRM7 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 7G8A2 unconjugated
anti-Glutamate Receptor, Metabotropic 7 (GRM7) (N-Term) antibodyGRM7 适用: 人, 大鼠, 小鼠, 犬, Cow, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 7 (GRM7) (N-Term) antibodyGRM7 适用: 人, 大鼠, 小鼠, 犬, 兔, Cow, 马, 猴, 小鸡 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 7 (GRM7) (Extracellular Domain) antibodyGRM7 适用: 人, 大鼠, 小鼠, 犬, 兔, Cow, 马, 猴, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 7 (GRM7) (C-Term) antibodyGRM7 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 7 (GRM7) (AA 377-390), (Extracellular), (N-Term) antibodyGRM7 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 7 (GRM7) (C-Term) antibodyGRM7 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 7 (GRM7) (C-Term) antibodyGRM7 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 7 (GRM7) (AA 220-410) antibodyGRM7 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 7 (GRM7) (pSer862) antibodyGRM7 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: ICC, IHC, IHC-P (6 - 16 μg/mL), WB
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))
- 别名
- GRM7 / MGLUR7 (GRM7 产品)
- 别名
- MGLUR7 antibody, GLUR7 antibody, GPRC1G antibody, MGLU7 antibody, 6330570A01Rik antibody, BB176677 antibody, C030018L03 antibody, E130018M02Rik antibody, Gpr1g antibody, Gprc1g antibody, mGluR7 antibody, glutamate metabotropic receptor 7 antibody, glutamate receptor, metabotropic 7 antibody, GRM7 antibody, Grm7 antibody
- 背景
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Name/Gene ID: GRM7
Subfamily: Metabotropic glutamate
Family: GPCR
Synonyms: GRM7, GLUR7, MGLU7, MGLUR7, GPRC1G - 基因ID
- 2917
- 途径
- Sensory Perception of Sound, cAMP Metabolic Process, Feeding Behaviour
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